Clonal analysis of progenitor cell commitment of granulocyte or macrophage production

Journal of Cellular Physiology
D Metcalf, A W Burgess

Abstract

Granulocyte-macrophage colony formation by C57BL bone marrow cells was initiated in agar cultures either by the granulocyte-macrophage stimulus, GM-CSF, or by the predominantly macrophage stimulus, M-CSF. After 24 hours, paired daughter cells of granulocyte-macrophage colony-forming cells (GM-CFC) were separated by micromanipulation and one cultured in GM-CSF, the other in M-CSF. From the differentiation pattern of the resulting colonies, irreversible commitment of some cells occurred during the first 24 hours and completion of the first cell division. A similar result was obtained using granddaughter cells present after 24 hours of incubation. However, when intact developing day 2 and day 3 clones were cross-transferred to GM-CSF or M-CSF recipient cultures, irreversible commitment was more obvious. Most M-CSF-initiated clones exhibited irreversible commitment to macrophage formation in GM-CSF cultures and a high proportion of GM-CSF-initiated clones continued to produce granulocyte progeny after transfer to M-CSF. The results indicated that GM-CSF and M-CSF can irreversibly commit the progeny of GM-CFC respectively to granulocyte or macrophage production. While for women GM-CFC this occurs within 24 hours and one cell divisio...Continue Reading

References

Jan 1, 1978·Cell and Tissue Kinetics·G Wagemaker, M F Peters
Apr 1, 1978·The Journal of Cell Biology·G R Johnson, A W Burgess
Oct 15, 1979·Experimental Cell Research·M HoriuchiY Ichikawa
Oct 1, 1974·Journal of Cellular Physiology·D MetcalfP W Kincade

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Citations

Jan 1, 1991·International Journal of Immunopharmacology·P Cornaglia-FerrarisR Stradi
Jan 1, 1989·Progress in Growth Factor Research·W P Steward, J H Scarffe
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M A CantrellS Gillis
Dec 23, 1999·Current Opinion in Hematology·J L Shepard, L I Zon
Jun 30, 1998·APMIS. Supplementum·J Olweus
Aug 26, 1998·Pigment Cell Research·G ThibaudeauP Gerard
Jul 11, 2009·Science·Michael A RiegerTimm Schroeder
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D Metcalf
Sep 4, 1999·In Vitro Cellular & Developmental Biology. Animal·A D ForsmanS K Chapes
Mar 12, 2008·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·A EroğluA E Unal
Jul 1, 1992·Baillière's Clinical Haematology·I Davis, G Morstyn
Jun 1, 1997·Baillière's Clinical Haematology·C J Eaves, A C Eaves
Nov 1, 1986·International Journal of Cell Cloning·H E Broxmeyer
Aug 15, 1982·International Journal of Cancer. Journal International Du Cancer·D Metcalf
Jan 1, 1988·Critical Reviews in Oncology/hematology·H E Broxmeyer, D E Williams
Apr 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P A FordP J O'Dwyer
Jul 15, 2016·Archivum Immunologiae Et Therapiae Experimentalis·Geoffrey BrownEwa Marcinkowska
Jul 22, 2014·Experimental Cell Research·Max EndeleTimm Schroeder
Dec 31, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Mervin C Yoder
Apr 11, 2018·Immunology and Cell Biology·Geoffrey BrownRhodri Ceredig
Jul 25, 2018·International Journal of Molecular Sciences·Geoffrey BrownPanagiotis Tsapogas
May 22, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Metcalf
Sep 1, 1982·Journal of Cellular Physiology·D Metcalf, S Merchav
Sep 11, 2009·Nature·Tariq Enver, Sten Eirik W Jacobsen
Nov 1, 1983·The Journal of Pathology·T M Dexter, T D Allen
May 29, 2002·Gene Therapy·A J WagersI L Weissman
Feb 1, 1987·Immunology and Cell Biology·D Metcalf
Jul 1, 1998·International Reviews of Immunology·W S Alexander
Mar 12, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Metcalf

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