Clonal deletion of thymic mature T cells induced by staphylococcal enterotoxin B in murine fetal thymus organ culture

European Journal of Immunology
Y AibaY Katsura

Abstract

The present study aims at investigating the intrathymic maturational stage of T cells at which clonal deletion can be induced. Staphylococcal enterotoxin B (SEB) was added to organ cultures of murine fetal thymus lobes at various time points of culture, and V beta 8-expressing cells were assayed on day 14. V beta 8 low-expressing (V beta 8lo) cells were reduced to 40-60% of the control receiving no SEB, though the reduction was ambiguous when SEB was given on day 13. In marked contrast, V beta 8 high-expressing (V beta 8hi) cells were virtually completely deleted in all groups including the group given SEB on day 13. Most of the V beta 8hi cells that were deleted by 24 h of treatment with SEB were shown to be of the CD4+8- mature phenotype, though CD4-8+V beta 8hi cells were also deleted. It was further shown that the thymic V beta 8hi CD4+8- cells recovered from organs cultured for 14 days without SEB responded to immobilized anti-V beta 8 monoclonal antibody, indicating that V beta 8hi cells, which were highly sensitive to clonal deletion, were functionally competent mature T cells. These results strongly suggest that the thymus is capable of eliminating all T cells recognizing antigen present in the thymus regardless of the ...Continue Reading

References

Feb 11, 1992·The Journal of Experimental Medicine·Y LiuC A Janeway
Feb 1, 1991·The Journal of Experimental Medicine·R P SékalyR S Geha
Jan 1, 1990·International Immunology·J Yagi, C A Janeway
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PayneH Hengartner
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O LeoJ A Bluestone
Apr 24, 1987·Cell·J W KapplerP Marrack

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Citations

Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hiromitsu HaraHiroki Yoshida

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