Abstract
The global spread of carbapenem-resistant Klebsiella pneumoniae (CR-Kp) has become a massive threat to human health. We investigated the clonal relatedness of CR-Kp strains in central Taiwan. CR-Kp strains were prospectively collected from inpatients referred to Chung Shan Medical University Hospital (CSMUH) during September 2011 to December 2015. The presence of carbapenemase genes, including blaKPC-2, blaVIM-1, blaNDM-1, and blaOXA-48, was analysed with polymerase chain reaction (PCR) and sequence determination. Clonal relatedness was determined by pulse-field gel electrophoresis and multilocus sequence typing. Capsule synthesis loci were typed based on the variation of the wzi gene. A total of 174 CR-Kp strains were collected. KPC-2 and OXA-48 were present in 63 (36.2%) and 22 (12.6%) CR-Kp strains, respectively. Two strains isolated in 2014 coproduced KPC-2 and OXA-48. Nearly all (98%) carbapenemase-producing K. pneumoniae strains belonged to the ST11 clone and could be further grouped into distinct sub-lineages. Intriguingly, the first sub-lineage, designated ST11-Clade I, contained all KPC-2 strains; OXA-48 strains were mostly included in the second sub-lineage, ST11-Clade II. Furthermore, a variation on the capsule synth...Continue Reading
Citations
Dec 19, 2018·Antimicrobial Agents and Chemotherapy·Lingbin ShuRong Zhang
Jul 26, 2019·Expert Review of Molecular Diagnostics·Yi-Chyi LaiPo-Ren Hsueh
May 13, 2020·Journal of Applied Microbiology·D ChenF Chen
Apr 10, 2019·Antimicrobial Agents and Chemotherapy·Yao-Chen WangYi-Chyi Lai
Oct 31, 2019·The Journal of Antimicrobial Chemotherapy·Min-Chi LuYi-Chyi Lai
Sep 26, 2019·Frontiers in Public Health·Yajie ZhaoJianming Cao
Jan 21, 2021·Infection and Drug Resistance·Bo ZhaoGaojue Wu
Mar 12, 2021·Environment International·Brigid HoobanDearbháile Morris
Apr 26, 2021·Journal of Global Antimicrobial Resistance·Zineb CherakJean-Marc Rolain
Apr 22, 2021·Infection and Drug Resistance·Min ZhangWei Huang
May 9, 2021·International Journal of Antimicrobial Agents·Chih-Cheng Lai, Wen-Liang Yu
Jun 23, 2021·Gut Pathogens·Tsui-Chin WangL Kristopher Siu
Jul 3, 2021·Journal of Personalized Medicine·Keh-Sen LiuMin-Chi Lu
May 5, 2020·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Ching-Hsun WangFeng-Yee Chang
Aug 18, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Yanmei SunXiaoli Cao
Oct 5, 2021·Gut Microbes·Ming-Ko ChiangYi-Chyi Lai
Dec 21, 2021·Frontiers in Public Health·Wenli LiaoTieli Zhou