Clonal dissemination of KPC-2 producing Klebsiella pneumoniae ST11 clone with high prevalence of oqxAB and rmtB in a tertiary hospital in China: results from a 3-year period

Annals of Clinical Microbiology and Antimicrobials
Li ChengKui Zhang

Abstract

Carbapenemase-producing Klebsiella pneumoniae (CPKP) strains have emerged as a major problem for healthcare systems. The aim of this study was to determine the circulating clones and analyze the clinical and molecular characteristics of CPKP in our hospital. A total of 74 carbapenemase producers collected from our hospital from 2012 to 2014 were analyzed for the prevalence of extended-spectrum β-lactamase (ESBLs), plasmid-mediated quinolone resistance genes (PMQRs), exogenously acquired 16S rRNA methyltransferase (16S-RMTase), and plasmid-mediated AmpC enzyme (pAmpCs) by PCR and DNA sequencing. The sequence types (STs) of the carbapenemase producers were analyzed by multi-locus sequence typing (MLST). And Pulsed-field gel electrophoresis (PFGE) was performed to investigate the genetic relationship of KPC-2 producing strains. Clinical data were retrieved from the medical records. KPC-2 (n = 72) was the predominant enzyme followed by NDM-1 (n = 2); The genes blaCTX-M, blaSHV, blaTEM-1, blaDHA-1, rmtB, armA, oqxA, oqxB, and qnrB were present in 29 (39.2 %), 27 (36.5 %), 46 (62.2 %), 2 (2.7 %), 25 (33.8 %), 1 (1.4 %), 60 (81.1 %) and 56 (75.7 %), 6 (8.1 %) isolates, respectively. MLST analysis revealed 10 different STs. The most do...Continue Reading

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Citations

Jan 11, 2018·Polish Journal of Microbiology·Monika MachulskaMarek Gniadkowski
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Jan 1, 2019·Journal of Chemotherapy·Hamid VaezFarzad Khademi
Mar 6, 2019·Antimicrobial Resistance and Infection Control·Jun LiHaihong Hao
Sep 5, 2019·Frontiers in Microbiology·Xiaoyan CuiHong Du
Feb 10, 2018·Frontiers in Microbiology·Eun-Jeong YoonSeok Hoon Jeong

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Methods Mentioned

BETA
PCR

Software Mentioned

BioNumerics
BLAST
- Pro
Chromas

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