Clonal expansion of hepatic progenitor cells and differentiation into hepatocyte-like cells

Development, Growth & Differentiation
Wenbin LiuZhongjun Wu

Abstract

Hepatic progenitor cells (HPCs) in adult liver are promising for treatment of liver diseases. A biliary-derived HPC population in adult mice has been characterized by co-expression of stem cell marker Sry (sex determining region Y)-box 9 (SOX9) and biliary marker cytokeratin 7 (CK7). However, isolation of these HPCs in adult healthy liver without any selection procedures remains a big challenge in this field. Here, by establishing a simple and efficient method to isolate and expand the CK7+ SOX9+ HPCs in vitro as clones, we acquired a stable and largely scalable cell source. The CK7+ SOX9+ progenitor cells were then further induced to differentiate into hepatocyte-like cells with expression of mature hepatocyte markers albumin (Alb) and hepatocyte nuclear factor 4 alpha (HNF4α), both in vitro and in vivo in the presence of hepatocyte growth factor (HGF) and fibroblast growth factor 9 (FGF9). Furthermore, we found that the HPCs are highly responsive to transforming growth factor-beta (TGF-β) signals. Collectively, we identified and harvested a CK7+ SOX9+ progenitor cell population from adult mouse liver by a simple and efficient approach. The exploration of this HPC population offers an alternative strategy of generating hepatoc...Continue Reading

References

Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z QiH Ueno
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Roger Williams
Feb 23, 2007·Molecular Biology of the Cell·Naoki TanimizuKeith E Mostov
Jul 31, 2007·Biochemical and Biophysical Research Communications·Marianne AntoinePaul Kiefer
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Jul 30, 2008·Cell·Joan Massagué
Oct 7, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Atsushi SuzukiHideki Taniguchi
Nov 11, 2008·The British Journal of Surgery·S MeraniA M J Shapiro
Jan 27, 2009·Gastroenterology·Mark A Zern
Jan 11, 2011·Nature Genetics·Meritxell Huch, Hans Clevers
May 14, 2011·Journal of the American Society of Nephrology : JASN·Bi SongSharon D Ricardo
Aug 3, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Vincenzo CardinaleLola Reid
Oct 19, 2011·Cell and Tissue Research·Steven Dooley, Peter ten Dijke
Oct 26, 2011·The Prostate·Sarah SalmElaine Lynette Wilson
Mar 1, 2012·Nature Reviews. Gastroenterology & Hepatology·Vincenzo CardinaleDomenico Alvaro
Oct 26, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Guanhua Xie, Anna Mae Diehl
Jul 18, 2014·Science Translational Medicine·Sangeeta N BhatiaIra J Fox
Aug 19, 2014·Cell Stem Cell·Kilangsungla YangerBen Z Stanger
Aug 26, 2014·Cellular Reprogramming·Kathy E O'NeillJonathan M W Slack
Oct 20, 2014·Nature Medicine·Carey L WatsonMichael A Helmrath
Apr 3, 2015·Blood·Ulrika Blank, Stefan Karlsson
Jul 21, 2015·Nature Cell Biology·Wei-Yu LuStuart J Forbes
Oct 3, 2015·Cell Stem Cell·Thomas G Bird, Stuart J Forbes
Feb 14, 2016·Archives of Toxicology·Claudia LuckertStefanie Hessel
Feb 18, 2016·Stem Cells International·Guido CarpinoEugenio Gaudio
Mar 15, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Domenico Alvaro, Eugenio Gaudio
Aug 25, 2016·Cellular Signalling·Rebecca VasconcellosRodrigo R Resende

❮ Previous
Next ❯

Citations

May 30, 2020·Bioengineering·Xiaoyu ZhaoHon Fai Chan
Apr 6, 2021·World Journal of Hepatology·Yana V KiselevaYuri O Zharikov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Yi-Ming ZhouGong-Ren Fan
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Agnieszka BanasTakahiro Ochiya
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Jeonghoon HeoSnorri S Thorgeirsson
© 2022 Meta ULC. All rights reserved