PMID: 2499876Jan 1, 1989Paper

Clonal growth of human prostatic epithelial cells is stimulated by fibroblasts

The Prostate
J N KabalinT A Stamey

Abstract

The interaction of fibroblasts with adult human prostatic epithelial cells was studied in vitro. Stimulation of epithelial cell proliferation, measured by clonal growth assay, was demonstrated when prostatic epithelial cells were grown in coculture with fibroblasts. Epithelial growth in cocultures with fibroblasts was greater than could be obtained in isolated culture in an "optimized," serum-free medium previously described. Fibroblasts were able to compensate for the deletion of several growth factors from this "optimized" medium, including epidermal growth factor and insulin, but were notably unable to replace bovine pituitary extract. Epithelial growth stimulation identical to that achieved in coculture was produced by medium conditioned by fibroblasts. Similar results were obtained by using fibroblasts of adult human prostate origin, adult human skin, human fetal lung fibroblasts (IMR-90), and mouse 3T3 cells. No difference in response was demonstrated between prostatic epithelial cells derived from normal or malignant tissues. Fixed fibroblast monolayers and extracellular matrix prepared from fibroblast cultures failed to stimulate prostatic epithelial growth. These results suggest that a soluble growth factor is secreted...Continue Reading

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Citations

Oct 12, 2000·The Prostate·D M Peehl, R G Sellers
Jan 1, 1992·Journal of Cellular Biochemistry. Supplement·L W ChungJ S Rubin
Nov 1, 1990·Journal of Cellular Physiology·E M ValveriusD S Salomon
Aug 27, 1998·In Vitro Cellular & Developmental Biology. Animal·D M PeehlS T Wong
Mar 15, 1996·International Journal of Radiation Oncology, Biology, Physics·B GeryJ B Little
Nov 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·B G Mobbs, I E Johnson
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·S DélosP M Martin
Jul 1, 1996·Urologic Oncology·D M Peehl
Feb 23, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Kumi ObaraIchiro Saito
Mar 1, 1995·Microscopy Research and Technique·T G PretlowT P Pretlow
Jul 16, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Julia T ArnoldMarc R Blackman
Apr 1, 1995·The Journal of Urology·M G WientjesJ L Au
Apr 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R A Gatenby
Feb 1, 1993·Cancer·C MyersM W Linehan
Mar 29, 2003·Prostate Cancer and Prostatic Diseases·G P BromfieldR G Bristow

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