PMID: 41246Sep 1, 1979

Clonal hybrid cell lines expressing cholinergic and adrenergic properties

Proceedings of the National Academy of Sciences of the United States of America
R HeumannB Hamprecht


Different cholinergic cell lines were fused with an adrenergic neuroblastoma cell line (N115-BU-8). Its fusion with a cholinergic neuroblastoma-glioma hybrid produced a "hybrid-hybrid" line containing cholinergic and adrenergic enzyme activities. Both activities were also present in subclones of this line. The presence of catecholamines in single cells was confirmed by microspectrofluorimetry. These results are discussed with respect to the possibility of a simultaneous synthesis of noradrenaline and acetylcholine in single cells. The cholinergic and adrenergic enzyme activities are influenced by cell density, by dexamethasone, and by conditioned medium.


Jan 19, 1988·European Journal of Pharmacology·G Reiser, B Hamprecht
May 1, 1989·Journal of Biochemical and Biophysical Methods·J ScheererP Muhn
Jan 1, 1996·Journal of Physiology, Paris·H UlrichH C Schaller
Jul 1, 1982·Journal of Neurochemistry·T GlaserB Hamprecht
Mar 24, 1998·Mechanisms of Development·I Hermans-BorgmeyerH C Schaller


Jun 6, 1977·Biochemical and Biophysical Research Communications·B CulverK N Prasad
Mar 1, 1978·Journal of Cellular Physiology·X O BreakefieldR Shiman
Jan 1, 1978·Annual Review of Neuroscience·P H Patterson
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E J FurshpanD D Potter
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D SchubertY Kidokoro
May 1, 1975·Biological Reviews of the Cambridge Philosophical Society·K N Prasad
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V KachelG Ruhenstroth-Bauer
Dec 1, 1977·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R HeumannB Hamprecht
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·X O Breakefield, M W Nirenberg
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J A Peterson, M C Weiss
Jan 1, 1974·Analytical Biochemistry·B Hamprecht, T Amano
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G Augusti-Tocco, G Sato

Related Concepts

Cholinergic Fibers
Central Neuroblastoma
Adrenergic Fibers
Catecholamines Measurement
Cell Hybridization
Norepinephrine Measurement
Glioblastoma Multiforme Pathway

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.