PMID: 41246Sep 1, 1979

Clonal hybrid cell lines expressing cholinergic and adrenergic properties

Proceedings of the National Academy of Sciences of the United States of America
R HeumannB Hamprecht

Abstract

Different cholinergic cell lines were fused with an adrenergic neuroblastoma cell line (N115-BU-8). Its fusion with a cholinergic neuroblastoma-glioma hybrid produced a "hybrid-hybrid" line containing cholinergic and adrenergic enzyme activities. Both activities were also present in subclones of this line. The presence of catecholamines in single cells was confirmed by microspectrofluorimetry. These results are discussed with respect to the possibility of a simultaneous synthesis of noradrenaline and acetylcholine in single cells. The cholinergic and adrenergic enzyme activities are influenced by cell density, by dexamethasone, and by conditioned medium.

References

Jan 19, 1988·European Journal of Pharmacology·G Reiser, B Hamprecht
May 1, 1989·Journal of Biochemical and Biophysical Methods·J ScheererP Muhn
Jan 1, 1996·Journal of Physiology, Paris·H UlrichH C Schaller
Jul 1, 1982·Journal of Neurochemistry·T GlaserB Hamprecht
Mar 24, 1998·Mechanisms of Development·I Hermans-BorgmeyerH C Schaller

Citations

Jun 6, 1977·Biochemical and Biophysical Research Communications·B CulverK N Prasad
Mar 1, 1978·Journal of Cellular Physiology·X O BreakefieldR Shiman
Jan 1, 1978·Annual Review of Neuroscience·P H Patterson
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E J FurshpanD D Potter
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D SchubertY Kidokoro
May 1, 1975·Biological Reviews of the Cambridge Philosophical Society·K N Prasad
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V KachelG Ruhenstroth-Bauer
Dec 1, 1977·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R HeumannB Hamprecht
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·X O Breakefield, M W Nirenberg
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J A Peterson, M C Weiss
Jan 1, 1974·Analytical Biochemistry·B Hamprecht, T Amano
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G Augusti-Tocco, G Sato

Related Concepts

Cholinergic Fibers
Central Neuroblastoma
Adrenergic Fibers
Catecholamines Measurement
Cell Hybridization
Neuroblastoma
Clone
Norepinephrine
Norepinephrine Measurement
Glioblastoma Multiforme Pathway

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