Clonal organization of proliferating spermatogonial stem cells in adult males of two species of non-human primates, Macaca mulatta and Callithrix jacchus

Biology of Reproduction
Jens EhmckeStefan Schlatt

Abstract

The present study examines the existence of clonogenic patterns in the proliferation and differentiation of spermatogonial stem cells in two species of non-human primates, the marmoset and the rhesus monkey. We developed a novel approach to detect proliferating spermatogonial clones in whole mounts of seminiferous tubules. Dual fluorescence labeling of bromodeoxyuridine and acrosin in conjunction with confocal microscopy allows the description of the clonogenic and spatial arrangement of proliferating spermatogonia at specific stages of the seminiferous epithelial cycle. Cross-sections of paraffin-embedded tissue were labeled by the same approach. For both monkey species we demonstrate the presence of proliferating spermatogonial clones of variable size at specific stages of the cycle of the seminiferous epithelium. Detailed analysis of the rhesus monkey reveals proliferating Apale spermatogonia at stages VII and IX of the cycle of the seminiferous epithelium, and of proliferating B spermatogonia at stages II, IV, VI, and XII. Proliferating Apale spermatogonia at stages VII and IX of the cycle are organized in pairs or quadruplets. B1 spermatogonia appear as quadruplets or eight-cell clones, and B2 spermatogonia as 8- or 16-cel...Continue Reading

References

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Citations

Feb 16, 2006·Reproduction, Fertility, and Development·Jens EhmckeStefan Schlatt
Apr 15, 2010·Development, Growth & Differentiation·Shosei Yoshida
Oct 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Jens Ehmcke, Stefan Schlatt
Nov 3, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Brian P HermannKyle E Orwig
Aug 24, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·S AlbertJ Gromoll
Dec 20, 2007·Growth Factors·Mahmoud HuleihelEitan Lunenfeld
Apr 3, 2010·Trends in Endocrinology and Metabolism : TEM·Tony M Plant
Feb 10, 2006·Annals of the New York Academy of Sciences·Tony M PlantGary R Marshall
Jan 19, 2012·Microscopy Research and Technique·Mateus R BegueliniEliana Morielle-Versute
Apr 2, 2014·Seminars in Cell & Developmental Biology·Stefan Schlatt, Jens Ehmcke
Sep 7, 2016·Stem Cells and Development·Makiko YamadaMarco Seandel
Jan 18, 2017·Molecular Human Reproduction·N NeuhausH R Schöler
Apr 17, 2018·Molecular Human Reproduction·A L Caldeira-BrantH Chiarini-Garcia
Aug 23, 2017·Development·Sara Di PersioElena Vicini
Aug 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Hiroki NakataShoichi Iseki
Feb 28, 2019·Human Reproduction Update·Swati SharmaNina Neuhaus
Feb 1, 2006·Human Reproduction Update·Jens EhmckeStefan Schlatt
Sep 22, 2017·Primate Biology·Swati SharmaStefan Schlatt

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

MagnaFire

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