PMID: 10530408Oct 26, 1999Paper

Clonal rearrangements in childhood and adult precursor B acute lymphoblastic leukemia: a comparative polymerase chain reaction study using multiple sets of primers

European Journal of Haematology
A H LiG Roos

Abstract

Ig heavy chain (IgH) and T-cell receptor (TCR) gene rearrangements were investigated by polymerase chain reaction (PCR) amplification of diagnostic tumour samples from 91 patients (57 children and 34 adults, with cut-off at age 16) with precursor B acute lymphoblastic leukemia (ALL). Using primers directed to the framework regions (FR) 1, 2 and 3 of the IgH gene, clonal IgH rearrangements were observed in 82, 58 and 58%, respectively, whereas clonality was presented in 45 and 27% using primers hybridising to the TCR delta and gamma genes. A combination of all five primer sets used resulted in 96% positive cases (children 100%, adults 88%). The frequency of clonal IgH rearrangements correlated to patient age with a significantly lower fraction of positive cases in the adult group. The concomitant usage of more than one V(H) family gene was similar for childhood and adult ALL, and an over-representation of V(H)6 rearrangements was found in childhood ALL. Twenty-five out of 91 cases (27%) displayed an oligoclonal pattern for either IgH or TCR gene rearrangements (children 37%, adults 12%). A comparative analysis of samples from different compartments was performed in 23 patients, and differences between two or three compartments w...Continue Reading

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Citations

Feb 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Forida Y MortuzaLetizia Foroni
Nov 4, 2004·European Journal of Haematology·Anna AleskogRichard Rosenquist
Feb 15, 2001·Journal of Hematotherapy & Stem Cell Research·V WelterlinP R Hénon
Jul 17, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Emma Flordal ThelanderSvetlana Lagercrantz
Dec 16, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mattias BerglundSvetlana Lagercrantz
Nov 6, 2009·European Journal of Haematology·Mahmoud R MansouriRichard Rosenquist
Jan 31, 2008·Leukemia & Lymphoma·Fiona CraigSteven Swerdlow
Jun 20, 2002·European Journal of Haematology·Mia ThorséliusRichard Rosenquist

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