Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 strains of human and porcine origin.

Journal of Clinical Microbiology
S FrankeH Karch

Abstract

Shiga-like toxin (SLT)-producing Escherichia coli (SLTEC) O101 has recently been associated with hemorrhagic colitis and hemolytic-uremic syndrome in humans. In this study, SLTEC O101 strains from humans and pigs were characterized for clonal relatedness by nucleotide sequence analysis of their slt genes, DNA finger-printing of genomic DNA, and determination of virulence factors. The slt genes of five E. coli O101 strains were cloned and sequenced. For all strains, the deduced amino acid sequences of the B subunits were identical to those of the SLT-IIe present in the classical SLTEC O139 strains that cause edema disease in pigs. The A subunit revealed more than 99% homology to that of SLT-IIe. DNA fingerprinting revealed a high degree of genetic relatedness between the human and porcine O101 isolates. None of the O101 strains investigated had virulence factors frequently found in porcine (F107 fimbriae or heat-stable or heat-labile enterotoxins) or human SLTEC strains (eaeA or enterohemorrhagic E. coli hemolysin). The absence of virulence factors typical of SLT-I- and SLT-II-producing E. Coli together with the presence of SLT-IIe, a toxin previously seen only in porcine E. coli, suggests a new pathogenic mechanism for E. coli ...Continue Reading

References

Aug 1, 1992·Journal of Clinical Microbiology·H Böhm, H Karch
Dec 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A ThomasB Rowe

❮ Previous
Next ❯

Citations

Jul 9, 2002·Letters in Applied Microbiology·Christine BürkE Märtlbauer
Nov 11, 2008·Foodborne Pathogens and Disease·Pina M FratamicoPaula J Fedorka-Cray
Jan 9, 2014·Animal Health Research Reviews·Marion TsengJulie A Funk
Mar 4, 2000·Applied and Environmental Microbiology·H SchmidtH Karch
Mar 7, 2006·Applied and Environmental Microbiology·Lutz GeueFranz J Conraths
Jul 28, 2010·Tropical Animal Health and Production·Thi Kim Nguyen OanhHenri De Greve
Jan 18, 2005·Journal of Dairy Science·H S Hussein, T Sakuma
Jul 5, 2012·Journal of Clinical Microbiology·Flemming ScheutzAlison D O'Brien
Jul 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F AllerbergerH Karch
Feb 18, 2003·Journal of Applied Microbiology·A M Ibekwe, C M Grieve
Jan 27, 2012·Glycobiology·Johannes MüthingHelge Karch
Aug 12, 2014·Applied and Environmental Microbiology·Marion TsengJulie A Funk
Mar 21, 2017·Environmental Science and Pollution Research International·Aayushi MalooAnirudh Ram
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.