Clonality analysis of B-lymphoid proliferations using the polymerase chain reaction

Cancer
M D LozanoW C Chan

Abstract

Polymerase chain reaction (PCR) based assays are becoming more reliable, simpler, and faster alternatives to traditional Southern blot hybridization (SBH) analysis for the detection of clonal immunoglobulin heavy chain (IgH) gene rearrangements. However, a variety of technical approaches have been reported with markedly different results. We analyzed the frozen tissue of 147 neoplastic and hyperplastic lesions on which SBH had previously been performed. Semi-nested and single-step PCR methods were compared. Consensus primers to the joining segments and the framework region (FR) III of the variable segments of the IgH gene were used. All PCR products were analyzed by polyacrylamide gel electrophoresis (PAGE). Thirteen samples were re-analyzed using a denaturing gradient gel electrophoresis (DGGE) system. The overall concordance between SBH and semi-nested PCR assays was 80.2%. In the non-Hodgkin's lymphoma (NHL) group, 75% of the cases with IgH rearrangements by SBH were found to be monoclonal by PCR. Regardless of type of lesion, 71.7% of the cases with IgH rearrangements by SBH were found to be clonal by PCR. The concordance between the semi-nested and single-step procedures was 87.1%. DGGE was helpful in clarifying the result...Continue Reading

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Citations

Jul 25, 2000·Archives of Disease in Childhood·J G Ocejo-VinyalsF Leyva-Cobián
Jun 25, 1998·Leukemia & Lymphoma·F VianelloF Dazzi
Jul 12, 2002·American Journal of Hematology·S PachmannR Munker
Nov 14, 2002·American Journal of Clinical Pathology·Lyle C LawnickiTimothy C Greiner
Feb 6, 2004·Pathology·Dominic V SpagnoloJenny Turner
Sep 5, 1998·Cancer Treatment Reviews·D W HammondM H Goyns
Aug 15, 2000·American Journal of Clinical Pathology·J P HigginsR A Warnke
Dec 29, 2000·Applied Immunohistochemistry & Molecular Morphology : AIMM·J D HarvellR A Warnke
Sep 4, 1999·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·M FödingerA Chott
Jun 1, 1997·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A AielloS Pilotti
Sep 1, 1996·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A TierensT C Greiner
Dec 3, 1999·Archives of Pathology & Laboratory Medicine·L J Medeiros, J Carr

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