Clonality and genetic profiles of drug-resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa

MicrobiologyOpen
Nolwazi L BhembeEzekiel Green

Abstract

In this study, we investigated the diversity of drug-resistant Mycobacterium tuberculosis isolates from families who own cattle in the Eastern Cape Province of South Africa using spoligotyping and mycobacterial interspersed repetitive-unit-variable number tandem repeat (MIRU-VNTR) typing. The Mycobacterium tuberculosis was investigated using MIRU-VNTR and the Mycobacterium tuberculosis families were evaluated using spoligotyping. Spoligotyping grouped 91% of the isolates into seven clusters, while 9% of the deoxyribonucleic acid (DNA) from TB isolates were unclustered from a total of 154 DNA used. Previously described shared types were observed in 89.6% of the isolates, with the Beijing family, SIT1, the principal genotype in the province, while the families T, SIT53 and X1, SIT1329 were the least detected genotypes. MIRU-VNTR grouped 81% of the isolates in 23 clusters while 19% were unclustered. A combination of the VNTR and spoligotyping grouped 79% of the isolates into 23 clusters with 21% unclustered. The low level of diversity and the clonal spread of drug-resistant Mycobacterium tuberculosis isolates advocate that the spread of TB in this study may be instigated by the clonal spread of Beijing genotype. The results from t...Continue Reading

References

Feb 14, 1996·JAMA : the Journal of the American Medical Association·P J BifaniB N Kreiswirth
Aug 7, 1999·The Journal of Infectious Diseases·D van SoolingenJ D van Embden
Feb 5, 2002·Journal of Clinical Microbiology·Malcolm D YatesStuart M Wilson
Jun 18, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christophe SolaNalin Rastogi
Jan 15, 2004·Emerging Infectious Diseases·Troels LillebaekKristin Kremer
Jul 21, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mohamed Abdel Aziz, Abigail Wright
Sep 20, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Séverine FerdinandNalin Rastogi
Oct 17, 2006·Clinical Microbiology Reviews·Barun MathemaBarry N Kreiswirth
May 18, 2007·Journal of Clinical Microbiology·Sahal A M Al-HajojNalin Rastogi
Nov 9, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Manormoney Pillay, A Willem Sturm
Oct 14, 2008·The Journal of Infectious Diseases·Jason R AndrewsUNKNOWN Tugela Ferry Care and Research Collaboration
Nov 20, 2008·Journal of Fish Diseases·F PourahmadR H Richards
Apr 21, 2009·Lancet·Abigail WrightUNKNOWN Global Project on Anti-Tuberculosis Drug Resistance Surveillance
Jul 16, 2010·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Won Sun ParkJin Han
Aug 27, 2010·Journal of Clinical Microbiology·Haiyan DongKanglin Wan
Jul 26, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Irene CerezoNalin Rastogi
Mar 1, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christophe DemayNalin Rastogi
Mar 17, 2012·European Journal of Medicinal Chemistry·Baptiste VillemagneNicolas Willand
Dec 4, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Maria Conceição MartinsLucilaine Ferrazoli
Apr 5, 2013·Journal of Clinical Microbiology·Ben J MaraisRobin M Warren
Jun 5, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Imane ChaouiMohammed El Mzibri
Feb 26, 2014·Emerging Infectious Diseases·Neel R GandhiN Sarita Shah
Nov 2, 2014·Asian-Australasian Journal of Animal Sciences·C L F KatiyatiyaA Mushunje

❮ Previous
Next ❯

Citations

Aug 7, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Nolwazi L Bhembe, Ezekiel Green

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

Excel
Mathema
GeneXpert

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
T C VictorR M Warren
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
E AktaşChristophe Sola
© 2022 Meta ULC. All rights reserved