Clonality assays and megakaryocyte culture techniques in essential thrombocythemia

Leukemia & Lymphoma
L YanJ Brière

Abstract

The development of techniques permitting in vitro growth of human megakaryocytes progenitors and more recently identification of the proto oncogene c-mpl (Mpl-R) and its ligand (Mpl-L) have created new opportunities for studying pathophysiology of E.T. Plasma or serum of E.T. patients was unable to overestimulate MK colony formation by normal bone marrow cells. Significant increases in circulating CFU MK in E.T. patients have been repeatedly observed while in E.T. marrow, due to inappropriate sampling, colony number was not significantly different from normal. Spontaneous colony formation is observed in approximately 100% bone marrow and 85% blood from E.T. patients. Spontaneous colony formation persisted in plasma clot assay without added plasma or serum and in serum free agar cultures but only at a slightly lower rate than in plasma clot. Spontaneous colony formation in culture condition without plasma and serum were never observed with normal bone marrow and blood. Spontaneous MK growth was observed in a higher proportion of E.T. patients than erythroid colony formation but both phenomenon can occur in about 50% of the patients. CFU MK colony formation disappeared in serum free cultures using highly purified CD 34 cells. MK ...Continue Reading

References

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Citations

Mar 12, 2002·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·Anne Greist
Dec 21, 2000·British Journal of Haematology·P Harrison
Jan 11, 2007·Orphanet Journal of Rare Diseases·Jean B Brière
Apr 28, 2000·Leukemia & Lymphoma·M GriesshammerH Schrezenmeier
Feb 1, 1997·Baillière's Clinical Haematology·J BriereE Peynaud-Debayle
Sep 25, 2004·American Journal of Hematology·Andreas KreftCharles James Kirkpatrick

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