Clonality investigation of clinical Escherichia coli isolates by polymerase chain reaction-based open-reading frame typing method

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
Masachika SaekiSatoshi Takahashi

Abstract

Escherichia coli (E. coli) causes urinary tract infections, pneumonia, surgical site infections, and bloodstream infections and is the important pathogen for both community-acquired and healthcare-associated infections. To investigate the clonality of E. coli is important for infection control and prevention. We aimed to investigate the clonality of clinical E. coli isolates using Cica Geneus E. coli polymerase chain reaction (PCR)-based open-reading frame typing (POT) KIT and clarify the clinical usefulness of this kit. About 124 E. coli isolates obtained from inpatients at Sapporo Medical University Hospital were used. The POT method was used to classify 124 clinical isolates into 87 POT numbers. In addition to the clonality, it was possible to obtain additional information that 20 of the 124 isolates were extended-spectrum β-lactamase (ESBL) producing E. coli (5 isolates of CTX-M-1 group and 15 isolates of CTX-M-9 group) and 13 were sequence type (ST) 131 clone. Furthermore, when these ESBL-producing 20 isolates were compared with pulsed-field gel electrophoresis (PFGE) or multilocus sequence typing (MLST), Simpson's index of diversity was 0.968 in POT method, 0.979 in PFGE, and 0.584 in MLST. POT method had an analytical po...Continue Reading

References

Jul 12, 2002·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Ling MaKeizo Yamaguchi
Dec 8, 2005·Journal of Clinical Microbiology·Sara Y TartofLee W Riley
Aug 19, 2008·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Ritsuko SatohFumitake Gejyo
Mar 30, 2013·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Hidenobu HidakaHiroshi Watanabe
Nov 29, 2013·BioMed Research International·Gabriele MessinaPietro Manzi
May 16, 2014·Journal of Clinical Microbiology·Masanori WatahikiTetsutaro Sata
Jul 15, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Kiyohito IshikawaYohei Matsuda
Aug 27, 2015·BMC Bioinformatics·Jónathan HerasMyriam Zarazaga
Dec 27, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Akihiro NakamuraSeiji Kawano
May 30, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Teppei SasaharaYuji Morisawa
Apr 11, 2017·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yoshio TakesueKatsunori Yanagihara
Jul 16, 2017·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yoshikazu IshiiHiroshi Kiyota
Aug 2, 2017·The Journal of Hospital Infection·A Kanayama KatsuseI Kobayashi
Feb 6, 2018·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Chika TakanoSatoshi Hayakawa
May 24, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Kumiko KawamuraYoshichika Arakawa
May 29, 2018·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yuto HarukiTetsuhiro Sugiyama

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