Clone and functional analysis of Seryl-tRNA synthetase and Tyrosyl-tRNA synthetase from silkworm, Bombyx mori

Scientific Reports
JingSheng HuBing Li

Abstract

Aminoacyl-tRNA synthetases are the key enzymes for protein synthesis. Glycine, alanine, serine and tyrosine are the major amino acids composing fibroin of silkworm. Among them, the genes of alanyl-tRNA synthetase (AlaRS) and glycyl-tRNA synthetase (GlyRS) have been cloned. In this study, the seryl-tRNA synthetase (SerRS) and tyrosyl-tRNA synthetase (TyrRS) genes from silkworm were cloned. Their full length are 1709 bp and 1868 bp and contain open reading frame (ORF) of 1485 bp and 1575 bp, respectively. RT-PCR examination showed that the transcription levels of SerRS, TyrRS, AlaRS and GlyRS are significantly higher in silk gland than in other tissues. In addition, their transcription levels are much higher in middle and posterior silk gland than in anterior silk gland. Moreover, treatment of silkworms with phoxim, an inhibitor of silk protein synthesis, but not TiO2 NP, an enhancer of silk protein synthesis, significantly reduced the transcription levels of aaRS and content of free amino acids in posterior silk gland, therefore affecting silk protein synthesis, which may be the mechanism of phoxim-silking disorders. Furthermore, low concentration of TiO2 NPs showed no effect on the transcription of aaRS and content of free amin...Continue Reading

Citations

Aug 31, 2000·Annual Review of Biochemistry·Michael Ibba, D Soll
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Tetsuo Asakura, Juming Yao
Oct 11, 2003·Biotechnology Advances·Yu-Qing Zhang
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Patrick O'Donoghue, Zaida Luthey-Schulten
Sep 17, 2005·Biochimie·Luc BonnefondJoëlle Rudinger-Thirion
Apr 22, 2009·Annual Review of Microbiology·Jiqiang LingMichael Ibba
Mar 17, 2011·Journal of Economic Entomology·Quan-You YuCheng Lu
Nov 26, 2013·Pesticide Biochemistry and Physiology·L MaBing Li
May 3, 2014·Biological Trace Element Research·Hua ZhangBing Li
Jan 2, 2015·Archives of Environmental Contamination and Toxicology·Ling WangFashui Hong
Jun 25, 2015·Biological Trace Element Research·YangYang LiBing Li

Related Concepts

Study
Alanine-tRNA Ligase Activity
Genes
Enzymes, antithrombotic
GARS gene
Amino Acids, I.V. solution additive
MT-TA gene
Transcription, Genetic
Bombyx
Inhibitors

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