Cloned T cells from a recent onset IDDM patient reactive with insulin B-chain

Journal of Autoimmunity
N C SchlootB O Roep

Abstract

Insulin-dependent diabetes mellitus (IDDM) results from selective autoimmune destruction of insulin producing beta-cells. T-cell reactivity and autoantibodies to several islet proteins such as insulin, GAD and IA-2 are associated with IDDM in mice and men. In NOD mice, the majority of T cells from insulitis specifically recognize the insulin B-chain peptide amino acid 9-22, in contrast to the periphery where the precursor frequency is much lower. It is important to note that these cells are diabetogenic. Surprisingly, the same insulin B-chain region contains epitopes recognized by protective T cells. In fact, autoimmune diabetes in NOD mice could be prevented by prophylactic treatment with this immunodominant T-cell epitope. In humans, however, no immunodominant regions of insulin have yet been defined. We have isolated and characterized a human insulin-specific T-cell clone that was derived from peripheral blood of a newly diagnosed IDDM patient. This patient displayed weakly positive primary T-cell responses to insulin. The peptide recognized by the clone was mapped to the insulin B chain (B:11-27). Functionally, the human insulin-specific CD4+ T cells displayed a Th1/0 like cytokine profile and were restricted by HLA-DR. The...Continue Reading

References

Oct 1, 1988·Diabetes·K HaskinsK Lafferty
Nov 16, 1995·Journal of Immunological Methods·H S de KosterJ W Drijfhout
Jun 1, 1995·Immunology Today·N Schloot, G S Eisenbarth
Nov 24, 1994·The New England Journal of Medicine·M A Atkinson, N K Maclaren
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·D Daniel, D R Wegmann
Jun 1, 1996·Diabetes·I Durinovic-BellòA G Ziegler
Mar 1, 1996·The Journal of Experimental Medicine·S M TompkinsP E Jensen
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·I BergerotC Thivolet
Oct 6, 1997·Diabetes/metabolism Reviews·N C Schloot, B O Roep

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Citations

Dec 21, 2011·The Journal of Biological Chemistry·Menno van LummelFrits Koning
May 9, 2012·Anatomy Research International·Norio KanatsunaAke Lenmark
Mar 24, 2004·Current Diabetes Reports·Constadina PanagiotopoulosRusung Tan
Mar 29, 2002·Diabetes/metabolism Research and Reviews·N C SchlootH Rothe
Sep 12, 2009·Journal of Autoimmunity·Christian PflegerUNKNOWN p520/521 Study Group
Mar 19, 2009·Clinical and Experimental Immunology·S I ManneringL C Harrison
Sep 16, 2015·Current Diabetes Reports·John W McGintyEddie A James
Apr 26, 2005·Journal of Immunological Methods·Stuart I ManneringLeonard C Harrison
Sep 10, 2005·Trends in Molecular Medicine·F Susan Wong
Oct 21, 2000·Journal of Autoimmunity·D R Wegmann, G S Eisenbarth
Apr 1, 2004·Endocrinology and Metabolism Clinics of North America·Timothy I M Tree, Mark Peakman
Nov 22, 2005·Clinical & Developmental Immunology·J M Jasinski, G S Eisenbarth
Aug 30, 2008·Aging & Mental Health·Simone Pettigrew, Michele Roberts
Oct 13, 2006·Current Opinion in Infectious Diseases·A D SawitzkeB C Cole
Mar 7, 2017·Nature Medicine·Maria J L KrachtBart O Roep
Sep 30, 2017·Frontiers in Endocrinology·Brittney N Newby, Clayton E Mathews
Apr 26, 2003·Journal of Pediatric Endocrinology & Metabolism : JPEM·Katarzyna KadzielaEwa Pańkowska
Oct 25, 2002·Diabetes/metabolism Research and Reviews·Guido MeierhoffNanette C Schloot

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