Cloning and analysis of a cDNA encoding a two-domain hemoglobin chain from the water flea Daphnia magna

Gene
Shin-ichi TokishitaHideo Yamagata

Abstract

A cDNA encoding a two-domain hemoglobin (Hb) chain of Daphnia magna was cloned and its nucleotide (nt) sequence of 1261 bp was determined. The nt sequence contained 74 bp of the leader sequence, 1047 bp of an open reading frame (ORF), and 119 bp of the 3'-untranslated region (UTR), excluding the polyadenylation tail. A sequence, AATACA, located 24 bp upstream from the polyA sequence was considered to be a polyadenylation signal. cDNA-derived amino acid (aa) sequence revealed that D. magna Hb chain is synthesized as a secretory precursor with a signal peptide of 18 aa. Mature D. magna Hb chain consists of 330-aa residues with a calculated molecular weight of 36227, which is composed of two large repeated domains, domain 1 and 2. Several key aa that are invariant in all or most of other Hb and required for functional heme-binding are conserved in each of the two domains. The N-terminal extension (pre-A segment) of domain 1 was unusually long and contained an unusual threonine-rich sequence. The homology between the aa sequences of the two domains (24% identity) was much lower than that observed in other two-domain Hb chains from clams or nematode. Hb mRNA level in D. magna reared under low oxygen concentration was more than 12 ti...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I De BaereM Perutz
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y NaitoA F Riggs
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B DixonB Pohajdak
Jul 20, 1989·Journal of Molecular Biology·H IwaasaK Shikama
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Sep 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S N VinogradovC N Trotman
Oct 1, 1995·Protein Science : a Publication of the Protein Society·O H KappS N Vinogradov

❮ Previous
Next ❯

Citations

Feb 6, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bettina ZeisRüdiger Jörg Paul
Jul 19, 2000·Genome Research·E BeaudoingD Gautheret
Nov 26, 2005·Biological Chemistry·Tobias LamkemeyerBettina Zeis
Apr 7, 2005·Journal of Inorganic Biochemistry·Andrew J GowJoseph Bonaventura
May 10, 2006·Biochemical and Biophysical Research Communications·Shin-Ichi TokishitaHideo Yamagata
Mar 3, 2007·Analytical Biochemistry·Edward DeMollAusten F Riggs
Jun 1, 2004·The Journal of Biological Chemistry·Thomas A GorrH Franklin Bunn
Jan 6, 2018·Evolutionary Applications·Maria Cuenca CambroneroLuisa Orsini
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov
Feb 2, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Peter GerkeRüdiger J Paul

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.