Cloning and analysis of the human complement factor H gene promoter.

Immunology and Cell Biology
H M WardD L Gordon

Abstract

The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.

References

Dec 1, 1992·Journal of Neuroscience Methods·D L GordonT A Sadlon
Jan 15, 1988·The Biochemical Journal·J RipocheR B Sim
Mar 25, 1995·Nucleic Acids Research·P D SiebertS A Lukyanov

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Citations

Feb 8, 2016·Archives of Gerontology and Geriatrics·Xuan LiaoQing-qing Tan
Jul 19, 2005·Molecular Immunology·Zhu-Zhu ChengT Sakari Jokiranta
Jun 10, 2009·Journal of Molecular Biology·Azubuike I OkemefunaStephen J Perkins
Feb 28, 2006·Survey of Ophthalmology·Larry A DonosoGregory Hageman
Jun 28, 2011·Advanced Drug Delivery Reviews·Maria V Carroll, Robert B Sim
Mar 19, 2005·Clinical Chemistry·Zhu-Zhu ChengT Sakari Jokiranta
Dec 6, 2016·The Journal of Clinical Investigation·Lindsay S KeirMartin Friedlander

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