PMID: 9171382Jun 1, 1997Paper

Cloning and analysis of the pepV dipeptidase gene of Lactococcus lactis MG1363

Journal of Bacteriology
M A HellendoornJ Kok

Abstract

The gene pepV, encoding a dipeptidase from Lactococcus lactis subsp. cremoris MG1363, was identified in a genomic library in pUC19 in a peptidase-deficient Escherichia coli strain and subsequently sequenced. PepV of L. lactis is enzymatically active in E. coli and hydrolyzes a broad range of dipeptides but no tri-, tetra-, or larger oligopeptides. Northern (RNA) and primer extension analyses indicate that pepV is a monocistronic transcriptional unit starting 24 bases upstream of the AUG translational start codon. The dipeptidase of L. lactis was shown to be similar to the dipeptidase encoded by pepV of L. delbrueckii subsp. lactis, with 46% identity in the deduced amino acid sequences. A PepV-negative mutant of L. lactis was constructed by single-crossover recombination. Growth of the mutant strain in milk was significantly slower than that of the wild type, but the strains ultimately reached the same final cell densities.

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Citations

Aug 23, 2003·The Journal of Biological Chemistry·Holger A LindnerRobert Ménard
Feb 2, 2020·Applied and Environmental Microbiology·Chenxi HuangJan Kok
Jan 25, 2005·Infection and Immunity·J M GoldsteinJ Travis
Sep 6, 2003·Applied and Environmental Microbiology·Joanna C WilkinsKaren A Homer
Apr 22, 2006·Applied Microbiology and Biotechnology·Kirsi SavijokiPekka Varmanen
Jun 6, 2003·Applied and Environmental Microbiology·Wilbert SybesmaJeroen Hugenholtz
Nov 5, 2019·Biochimica Et Biophysica Acta. General Subjects·Saleem Yousuf Bhat, Insaf Ahmed Qureshi
May 9, 1998·Applied and Environmental Microbiology·P VarmanenS Tynkkynen

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