PMID: 2508062Sep 25, 1989Paper

Cloning and analysis of the spc ribosomal protein operon of Bacillus subtilis: comparison with the spc operon of Escherichia coli

Nucleic Acids Research
T M HenkinM Nomura

Abstract

A segment of Bacillus subtilis chromosomal DNA homologous to the Escherichia coli spc ribosomal protein operon was isolated using cloned E. coli rplE (L5) DNA as a hybridization probe. DNA sequence analysis of the B. subtilis cloned DNA indicated a high degree of conservation of spc operon ribosomal protein genes between B. subtilis and E. coli. This fragment contains DNA homologous to the promoter-proximal region of the spc operon, including coding sequences for ribosomal proteins L14, L24, L5, S14, and part of S8; the organization of B. subtilis genes in this region is identical to that found in E. coli. A region homologous to the E. coli L16, L29 and S17 genes, the last genes of the S10 operon, was located upstream from the gene for L14, the first gene in the spc operon. Although the ribosomal protein coding sequences showed 40-60% amino acid identity with E. coli sequences, we failed to find sequences which would form a structure resembling the E. coli target site for the S8 translational repressor, located near the beginning of the L5 coding region in E. coli, in this region or elsewhere in the B. subtilis spc DNA.

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Citations

Nov 1, 1990·Molecular & General Genetics : MGG·C B MichalowskiH J Bohnert
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·M A Hayat, D A Mancarella
Feb 11, 1993·Nucleic Acids Research·Y L ChanI G Wool
May 8, 1991·European Journal of Biochemistry·O JahnV A Erdmann
Aug 20, 2004·Journal of Bacteriology·Todd D AllenJanice M Zengel
Aug 15, 1991·Biochemical and Biophysical Research Communications·Y L ChanI G Wool
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J M ZengelL Lindahl
Feb 1, 1995·Biological Chemistry Hoppe-Seyler·P Tsiboli, T Choli
Aug 1, 1992·European Journal of Biochemistry·S HerwigB Wittmann-Liebold
Jan 1, 1994·Journal of Bacteriology·B Arnold-Schulz-GahmenF Le Hégarat
Sep 1, 1990·Journal of Bacteriology·A M Albertini, A Galizzi

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