Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY

Gene
F NiehausG Antranikian

Abstract

The gene for an extremely thermostable DNA polymerase has been cloned from chromosomal DNA of the recently characterised hyperthermophilic archaeon Thermococcus sp. TY by using degenerate primers derived from consensus sequences of known archaeal enzymes. The corresponding enzyme was overexpressed in Escherichia coli. Sequence comparison of the gene with related DNA polymerase genes revealed that it is interrupted by three regions showing high similarities to self-splicing protein elements, so-called "inteins". This is the first DNA polymerase containing such a large number of self-splicing elements. To ensure an efficient expression, these regions were deleted on the DNA level. The resulting protein showed DNA polymerase and 3'-5' exonuclease activity at high temperatures, being a promising candidate for use in the polymerase chain reaction (PCR).

References

Oct 25, 1992·Nucleic Acids Research·J HainW Zillig
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F B PerlerS K Hempstead
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K MullisH Erlich
Feb 25, 1993·Nucleic Acids Research·D K Braithwaite, J Ito
Jul 1, 1995·Bio/technology·M W AdamsR M Kelly
Jun 29, 2012·Brain : a Journal of Neurology·Janel O JohnsonAndrew B Singleton
Jan 1, 1995·World Journal of Microbiology & Biotechnology·M CiaramellaM Rossi

❮ Previous
Next ❯

Citations

Aug 2, 2008·Extremophiles : Life Under Extreme Conditions·Damien MarsicJoseph D Ng
Aug 4, 2001·Trends in Genetics : TIG·S Pietrokovski
Jul 8, 2005·Applied and Environmental Microbiology·Keizo NagasakiShmuel Pietrokovski
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Jerry Eichler, Michael W W Adams
Aug 5, 2009·Environmental Microbiology·Karen D WeynbergWilliam H Wilson
Sep 20, 2006·Protein Expression and Purification·Kate GriffithsPeter L Bergquist
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Dae Jin KimYu Sam Kim
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A J MacarioE Conway de Macario

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.