Abstract
The beta-1,4-mannanase 5C gene (man5C) of Vibrio sp. strain MA-138 was cloned and expressed in Escherichia coli. The man5C gene consisted of 2,010 bp nucleotides encoding a protein of 669 amino acids with a predicted molecular weight of 76,309. beta-1,4-Mannanase (Man5C) is a modular enzyme composed of a catalytic module belonging to glycoside hydrolase family 5, a linker region, and a putative carbohydrate-binding module (CBM) belonging to family 27. Recombinant Man5C exhibited maximal activity at 50 degrees C at pH 7.0, and it had a K(m) of 0.6 mg ml(-1) and a V(max) of 556.2 micromol min(-1) mumol(-1) for glucomannan. Binding studies revealed that the C-terminal putative CBM27 had the ability to bind soluble beta-mannans and contributed to increasing the rate of depolymerization by binding to the polymeric substrate. Man5C of Vibrio sp. MA-138 is the first non-extremophile enzyme to be identified as a beta-mannanase possessing CBM27.
References
Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Aug 15, 1970·Nature·U K Laemmli
Sep 5, 1995·Biochemistry·N SugimotoM Sasaki
Aug 1, 1995·Enzyme and Microbial Technology·B R AliR B Freedman
Jan 9, 1998·Planta·J D BewleyG B Fincher
Feb 17, 2000·FEMS Microbiology Letters·D StollR A Warren
Jun 8, 2001·The Biochemical Journal·A SunnaP L Bergquist
Sep 19, 2001·The Journal of Biological Chemistry·A C FreeloveH J Gilbert
Oct 9, 2001·Biotechnology and Bioengineering·K N ParkerR M Kelly
Jan 14, 2003·The Biochemical Journal·Deborah HoggHarry J Gilbert
Jun 7, 2003·Structure·Alisdair B BorastonGideon J Davies
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Jul 2, 1960·Nature·Y IRIKIT MIWA
Mar 9, 2004·The Journal of Biological Chemistry·David N BolamHarry J Gilbert
Jun 24, 2004·Journal of Molecular Biology·Yvette RoskeUdo Heinemann
Aug 19, 2004·Extremophiles : Life Under Extreme Conditions·Yanhe MaPeijin Zhou
Jul 7, 2005·Extremophiles : Life Under Extreme Conditions·Yuji HatadaKoki Horikoshi
Jun 1, 1948·Journal of the American Chemical Society·E HEYNE, R L WHISTLER
Citations
May 4, 2012·Journal of Experimental Botany·María del Carmen Rodríguez-GacioAngel J Matilla
May 26, 2009·Marine Biotechnology·Yoshiaki Umemoto, Toshiyoshi Araki
Nov 16, 2011·Biotechnology for Biofuels·João Paulo L Franco CairoFabio M Squina
Feb 9, 2012·Applied Microbiology and Biotechnology·Prakram Singh ChauhanNaveen Gupta
Nov 25, 2011·Enzyme and Microbial Technology·Do Young KimHo-Yong Park
Oct 23, 2015·Folia Microbiologica·Rui ZhangZunxi Huang
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Yoshihiro HakamadaShinichi Ohashi
Dec 21, 2016·Biological Reviews of the Cambridge Philosophical Society·Simon LadevèzeGabrielle Potocki-Véronèse
Oct 25, 2016·Journal of Bioscience and Bioengineering·Jianfang LiMinchen Wu
Sep 30, 2009·Applied Microbiology and Biotechnology·Anwar Sunna
Oct 9, 2019·The FEBS Journal·Andrea StrazzulliMarco Moracci
Jan 26, 2020·Applied and Environmental Microbiology·Isaac CannRoderick I Mackie
Oct 10, 2020·Critical Reviews in Biotechnology·Gaurav Singh Kaira, Mukesh Kapoor
Nov 5, 2020·International Journal of Biological Macromolecules·Xi ChenKui Wang
Jan 16, 2010·Journal of Agricultural and Food Chemistry·Yi-Min HsiaoYi-Hsiung Tseng