PMID: 9171081Jun 15, 1997Paper

Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium album

Nucleic Acids Research
A WeiheT Börner

Abstract

We have cloned a full-length cDNA from the higher plant Chenopodium album coding for a single subunit bacteriophage-type RNA polymerase. The cDNA isolated from an actively growing cell suspension culture recognized a 3.8 kb transcript on Northern blots. The open reading frame comprises 987 amino acids with a predicted molecular mass of 112 kDa. A comparison of the protein sequence with those of the two known fungal mitochondrial RNA polymerases, from Saccharomyces cerevisiae and Neurospora crassa , reveals extensive homology between the three enzymes. with complete conservation of all catalytically essential amino acids. The putative mitochondrial RNA polymerase from C.album , as well as homologous sequences from rice and barley, which have been partially cloned, lack two catalytically non-essential regions of up to 176 amino acids near the C-terminus present in the two fungal mitochondrial RNA polymerases. The extreme N-terminus of the cloned C.album RNA polymerase displays features of a potential mitochondrial transit sequence. In phylogenetic trees constructed to compare the evolutionary relationships between the different single subunit RNA polymerases the C.album sequence forms a subgroup together with the S.cerevisiae and...Continue Reading

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Citations

Nov 19, 2002·Biochemical and Biophysical Research Communications·Yuki KobayashiMamoru Sugita
Jul 20, 2002·Journal of Molecular Biology·Michaela Hoffmann, Stefan Binder
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·B HedtkeA Weihe
Jun 14, 2002·The Plant Journal : for Cell and Molecular Biology·Boris HedtkeThomas Börner
Mar 22, 2001·EMBO Reports·B HedtkeA Weihe
Oct 17, 2008·Molecular Biology and Evolution·Vadim V GoremykinRoberto Viola
Mar 21, 1998·Nucleic Acids Research·M W GrayG Burger
Jan 20, 2006·Nucleic Acids Research·Jacinthe AzevedoSilva Lerbs-Mache
May 20, 1999·The Plant Cell·C C ChangD B Stern
Sep 22, 2001·Protein Science : a Publication of the Protein Society·F D SchubotB C Wang
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Kyoko BabaRishikesh P Bhalerao
Apr 17, 2004·The Plant Journal : for Cell and Molecular Biology·Carola EmanuelThomas Börner
Nov 26, 2009·The Plant Journal : for Cell and Molecular Biology·Uwe RichterThomas Börner
Mar 22, 2014·PloS One·P Markus WilkenBrenda D Wingfield
Apr 3, 1999·The Journal of Biological Chemistry·S DombrowskiS Binder
Nov 24, 1999·Molecular and Cellular Biology·T M Ikeda, M W Gray
Mar 10, 1998·Plant Physiology·F HatzackS Binder

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