Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1): tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development

The Journal of Steroid Biochemistry and Molecular Biology
Yukinori KazetoKohei Yamauchi

Abstract

Cholesterol side-chain cleavage cytochrome P450 (CYP11A1: P450scc) is a crucial steroidogenic enzyme that catalyzes an initial step in the production of all classes of steroids. A cDNA encoding Japanese eel P450scc was cloned and characterized. The cDNA putatively encoded 521 amino acid residues with high homology to those of other vertebrate forms. The recombinant P450scc produced in COS-7 cells efficiently catalyzed the conversion of 25-hydroxycholesterol into pregnenolone. By northern blot, a single P450scc transcript of approximately 3.3 kb was detected in both ovary and head kidney. Transcript levels of this enzyme significantly increased throughout ovarian development artificially induced by salmon pituitary homogenate, which suggests that gonadotropic stimuli can induce ovarian expression of the P450scc gene in teleosts, as has been reported in mammals. Furthermore, RT-PCR analysis revealed that gene expression of three steroidogenic enzymes, P450scc, P450c17 and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) show distinctly different tissue-specific patterns of expression in the Japanese eel. The P450scc gene was expressed in ovary and head kidney while the sole source of the P450c17 transcript was ovary. In contrast, 3...Continue Reading

References

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Mar 6, 2003·Gene Expression Patterns : GEP·Hwei-Jan HsuBon-chu Chung
Jun 12, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Yukinori KazetoKohei Yamauchi

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Citations

Jun 12, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Yukinori KazetoKohei Yamauchi
Sep 22, 2009·General and Comparative Endocrinology·Arjan P PalstraGuido E E J M van den Thillart
Aug 12, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anbazhagan Rajakumar, Balasubramanian Senthilkumaran
Jan 16, 2020·Fish Physiology and Biochemistry·Anbazhagan Rajakumar, Balasubramanian Senthilkumaran
Nov 21, 2019·Molecular Reproduction and Development·Lihui MengXubo Wang
Sep 12, 2018·International Journal of Molecular Sciences·Dongdong LiangFeng You
Dec 4, 2020·General and Comparative Endocrinology·Hiroshi SuzukiYukinori Kazeto
May 21, 2021·Frontiers in Neuroendocrinology·Yiqi Christina Lin, Vassilios Papadopoulos
Mar 19, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Yukinori KazetoShinji Adachi
Mar 16, 2012·Environmental Toxicology and Pharmacology·Tomohide UnoTakao Itakura

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