Cloning and characterization of a cDNA encoding human differentiation antigen 5D4

Science in China. Series C, Life Sciences
F MaX Wang

Abstract

A 1 846 bp cDNA is isolated from a human tonsil cell lambda gt 11 cDNA library (ATCC No. 37546) with mAb 5D4 reactive strongly with human B cell line 3D5, but weakly with human B cell line Daudi and human T cell line Jurkat as a probe. RT-PCR also shows a strong reaction in 3D5 cell and a weak reaction in Daudi and Jurkat cell for 5D4 mRNA. There is an open reading frame from 88 to 1 209 bp in 5D4 cDNA encoding a 374 AA protein. Both the Northern blot analysis and the two consecutive stop codens before start coden demonstrate that the cDNA is a full-length cDNA. Secondary structure prediction suggests that there are a region from 295 to 334 AA in the protein with strong hydrophobicity and a transmembrane helix region with high score from 313 to 334 AA with an orientation from the inside to the outside of the cell.

References

Oct 1, 1989·British Journal of Haematology·D A Triplett, J Brandt
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jul 1, 1988·European Journal of Biochemistry·G von Heijne, Y Gavel
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M SinghA G Cochran

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Citations

Sep 14, 2001·Scandinavian Journal of Immunology·G X ShiL P Zhu

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