Cloning and characterization of a cDNA encoding the wheat (Triticum durum Desf.) CM16 protein

Plant Molecular Biology
M F GautierP Joudrier

Abstract

A Triticum durum cDNA library prepared from developing endosperm (22 days after flowering (DAF] was screened using synthetic oligonucleotide probes covering part of the CM3 and CM16 N-terminal protein sequences. A full-length cDNA clone (pTd78) encoding the CM16 protein (chloroform/methanol-soluble protein) was isolated and characterized. To our knowledge this is the first characterization of a clone coding for a wheat CM protein. The CM16 protein is synthesized as a preprotein with a signal peptide of 24 residues, the molecular weight of the mature protein being 13,438 Da. As other members of the cereal trypsin/alpha-amylase inhibitor family, the CM16 protein contains 10 cysteine residues, their position being well conserved. In developing endosperm the highest level of CM16 mRNA was detected at mid-maturation.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Jan 17, 1986·Biochimica Et Biophysica Acta·D BarberG Salcedo
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Apr 29, 1985·Biochimica Et Biophysica Acta·K MaedaH Matsubara
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Feb 28, 1983·Biochimica Et Biophysica Acta·K MaedaH Matsubara
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L GomezG Salcedo
Oct 1, 1987·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R Sanchez-MongeG Salcedo
Mar 1, 1986·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J Paz-AresP Carbonero
Dec 1, 1984·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P Fra-MonF García-Olmedo
May 1, 1984·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G SalcedoF García-Olmedo
May 1, 1984·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J HejgaardG Nielsen
Jan 1, 1975·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C AragoncilloF Garcia-Olmedo

❮ Previous
Next ❯

Citations

Mar 16, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Kusaba-NakayamaK Imaizumi
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·J IulekM F Grossi de Sá
Jan 1, 1996·Critical Reviews in Food Science and Nutrition·R K Bush, S L Hefle
Mar 1, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hong Wei WangCheol Seong Jang
Nov 1, 1993·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·G A StewartA S McWilliam
Feb 6, 2002·Bioscience, Biotechnology, and Biochemistry·H YamashitaH Tsuji
Aug 3, 2005·Biochimica Et Biophysica Acta·Freddy BoutrotMarie-Françoise Gautier
Jan 1, 1995·DNA Sequence : the Journal of DNA Sequencing and Mapping·P JoudrierM F Gautier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.