Cloning and characterization of a Chlamydia trachomatis L3 DNA fragment that codes for an antigenic region of the major outer membrane protein and specifically hybridizes to the C- and C-related-complex serovars.

Infection and Immunity
E J CarlsonL M de la Maza

Abstract

Chlamydia trachomatis L3 DNA was cloned and expressed in lambda gt11. A recombinant plaque that expressed an antigen that reacted with rabbit polyclonal antichlamydial L3 serum and with two monoclonal antibodies specific for serovars L3 and I was selected from this Chlamydia genomic library. The beta-galactosidase Chlamydia fusion protein was purified by immunoaffinity chromatography and injected into mice to produce monoclonal antibodies. These monoclonal antibodies reacted by Western (immuno-) blot with both the fusion protein and the major outer membrane protein from purified L3 elementary bodies. The chlamydial DNA fragment was shown by DNA sequence analysis to be 168 base pairs in length and to correspond to the constant regions 1 and 2 and the variable segment 1 of the major outer membrane protein gene. The recombinant chlamydial DNA fragment hybridized under stringent conditions by Southern and dot blot analysis exclusively with the DNA from the C- and C-related-complex C. trachomatis serovars.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1977·Journal of Clinical Microbiology·K T Ripa, P A Mårdh
Apr 1, 1975·Journal of Clinical Microbiology·S J Richmond, E O Caul
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J NovotnýG D Rose
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W BaehrH D Caldwell
Apr 1, 1988·Journal of Clinical Microbiology·E M Peterson, L M de la Maza
Sep 1, 1987·Journal of Bacteriology·R S StephensM S Urdea
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T TanakaM J Cline
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A Young, R W Davis
Aug 1, 1983·Infection and Immunity·E M Peterson, L M de la Maza
Sep 1, 1983·The Medical Clinics of North America·L M de la Maza, E M Peterson
Sep 1, 1984·Infection and Immunity·I AllanM A Lovett
Mar 1, 1982·Infection and Immunity·H D Caldwell, J Schachter
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

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