Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library

Extremophiles : Life Under Extreme Conditions
Charles C LeeDominic W S Wong

Abstract

There is a great interest in xylanases due to the wide variety of industrial applications for these enzymes. We cloned a xylanase gene (xyn8) from an environmental genomic DNA library. The encoded enzyme was predicted to be 399 amino acids with a molecular weight of 45.9 kD. The enzyme was categorized as a glycosyl hydrolase family 8 member based on sequence analysis of the putative catalytic domain. The purified enzyme was thermolabile, had an activity temperature optimum of 20 degrees C on native xylan substrate, and retained significant activity at lower temperatures. At 4 degrees C, the apparent K (m) was 3.7 mg/ml, and the apparent k (cat) was 123/s.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1989·Critical Reviews in Biotechnology·O P Ward, M Moo-Young
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch
Jan 1, 1997·Advances in Applied Microbiology·P Bajpai
Jul 25, 1998·Biochemistry and Molecular Biology International·K H YoonK H Jung
Feb 17, 2000·Trends in Biotechnology·C GerdayG Feller
Jul 6, 2000·Extremophiles : Life Under Extreme Conditions·I PetrescuC Gerday
Dec 29, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M TurkiewiczS Bielecki
Sep 11, 2001·Applied Microbiology and Biotechnology·Q K BegG S Hoondal
Mar 9, 2002·Journal of Molecular Biology·Diego M A GuérinPedro M Alzari
Apr 18, 2002·Critical Reviews in Biotechnology·S Subramaniyan, P Prema
Jun 29, 2002·The Journal of Biological Chemistry·Tony CollinsCharles Gerday
Jun 9, 2004·Applied and Environmental Microbiology·YaLi BrennanBrian A Steer
Aug 7, 2004·Current Opinion in Biotechnology·Wolfgang R StreitKarl-Erich Jaeger
Jan 15, 2005·Applied Microbiology and Biotechnology·Lambertus A M van den BroekAlphons G J Voragen
Jan 18, 2005·FEMS Microbiology Reviews·Tony CollinsGeorges Feller
Feb 2, 2006·Current Microbiology·Charles C LeeDominic W S Wong

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Citations

Mar 31, 2007·Applied Microbiology and Biotechnology·Christel SchmeisserWolfgang R Streit
Jun 26, 2009·Applied Microbiology and Biotechnology·Junpei ZhouBin Yao
Sep 18, 2009·Applied Microbiology and Biotechnology·Carola Simon, Rolf Daniel
Apr 16, 2010·Applied Microbiology and Biotechnology·Guozeng WangBin Yao
Feb 19, 2011·Extremophiles : Life Under Extreme Conditions·Skander ElleucheGarabed Antranikian
Jun 26, 2013·Extremophiles : Life Under Extreme Conditions·Eiji OhmaeChiaki Kato
Apr 12, 2011·Journal of Industrial Microbiology & Biotechnology·Indira P SarethySanjay Gupta
Feb 9, 2011·Applied Biochemistry and Biotechnology·Peng ZhouZhengqiang Jiang
Aug 3, 2011·Journal of Agricultural and Food Chemistry·Emmie DornezChristophe M Courtin
Jan 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jan SchoepeChristophe M Courtin
Aug 17, 2010·Applied and Environmental Microbiology·Shengguo ZhaoZhongtang Yu
May 20, 2009·Biotechnology for Biofuels·Luen-Luen LiDaniel van der Lelie
Jan 28, 2014·Extremophiles : Life Under Extreme Conditions·Xiaoshuang LiuZiduo Liu
May 25, 2010·Biotechnology Advances·In Taek HwangNo-Joong Park
Feb 15, 2012·The Biochemical Journal·Douglas B JordanKurt Wagschal
Mar 17, 2010·Critical Reviews in Biotechnology·Annick PolletChristophe M Courtin
Nov 19, 2013·Biotechnology Advances·Stijn LagaertGuido Volckaert
Nov 17, 2014·Extremophiles : Life Under Extreme Conditions·Jan Kjølhede VesterPeter Stougaard
Jan 1, 2013·Biology·Swati Joshi, Tulasi Satyanarayana
Jul 29, 2018·World Journal of Microbiology & Biotechnology·Zhenggang HanJiangke Yang
Sep 16, 2018·Applied and Environmental Microbiology·Xiu-Lan ChenPing-Yi Li
Jul 8, 2011·Microbial Biotechnology·R CavicchioliT J Williams
Aug 18, 2017·Applied Biochemistry and Biotechnology·Aravind MadhavanAshok Pandey
Jul 16, 2008·Journal of Agricultural and Food Chemistry·Mendel Friedman, Carol E Levin

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