PMID: 9547260Jun 20, 1998Paper

Cloning and characterization of a human DEAH-box RNA helicase, a functional homolog of fission yeast Cdc28/Prp8

Nucleic Acids Research
O ImamuraY Furuichi

Abstract

During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We have cloned a new RNA helicase gene, designated DBP2 (DEAH-boxprotein), homologous to the Schizosaccaromyces pombe cdc28(+)/prp8(+) gene involved in pre-mRNA splicing and cell cycle progression. The full-length DBP2 contains 3400 nucleotides and codes for a protein of 1041 amino acids with a calculated mol. wt of 119 037 Da. Transfection experiments demonstrated that the GFP-DBP2 gene product, transiently expressed in HeLa cells, was localized in the nucleus. The DBP2 gene was mapped by FISH to the MHC region on human chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Because the expression of DBP2 gene in S.pombe prp8 mutant cells partially rescued the temperature-sensitive phenotype, we conclude that DBP2 is a functional human homolog of the fission yeast Cdc28/Prp8 protein.

References

Dec 1, 1992·Trends in Biochemical Sciences·E V Koonin
Jan 1, 1991·Annual Review of Cell Biology·M R Green
Apr 1, 1991·Genes & Development·E J Strauss, C Guthrie
Jan 1, 1990·Annual Review of Biochemistry·S W Matson, K A Kaiser-Rogers
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 1, 1989·The Journal of Cell Biology·M Kozak
May 5, 1988·Nature·A E GorbalenyaV M Blinov
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J E Arenas, J N Abelson
Dec 6, 1997·Biochemical and Biophysical Research Communications·O ImamuraY Furuichi

❮ Previous
Next ❯

Citations

Oct 29, 2003·Journal of Immunological Methods·Marcia SticklerFiona A Harding
Jun 5, 2003·Genomics·Mohamed AbdelhaleemHester Wain
Apr 28, 2000·The International Journal of Biochemistry & Cell Biology·U Bond, T C James
Sep 16, 2010·The Journal of Biological Chemistry·Marieta GenchevaRen-Jang Lin
Jun 30, 2006·Nucleic Acids Research·Sergio de la Fuente van BentemHeribert Hirt
Apr 21, 2005·RNA·Richard J Grainger, Jean D Beggs
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·S W Stevens, J Abelson
Apr 29, 2010·The Biochemical Journal·Marieta GenchevaRen-Jang Lin
May 25, 2002·Experimental Cell Research·Benigno C ValdezDale Henning
Aug 28, 2012·RNA Biology·Roman MartinMarkus T Bohnsack
Mar 14, 2003·Journal of Andrology·Matthew D AnwayMichael D Griswold

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.