Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase: an enzyme that preferentially regulates metabolism of very long chain ceramides

The Journal of Biological Chemistry
Cungui MaoLina M Obeid

Abstract

Ceramidases deacylate ceramides, important intermediates in the metabolic pathway of sphingolipids. In this study, we report the cloning and characterization of a novel mouse alkaline ceramidase (maCER1) with a highly restricted substrate specificity. maCER1 consists of 287 amino acids, and it has a 28 and 32% identity to the Saccharomyces alkaline ceramidases (YPC1p and YDC1p) and the human alkaline phytoceramidase, respectively. Reverse transcriptase-PCR analysis demonstrated that maCER1 was predominantly expressed in skin. maCER1 was localized to the endoplasmic reticulum as revealed by immunocytochemistry. In vitro biochemical characterization determined that maCER1 hydrolyzed D-erythro-ceramide exclusively but not D-erythro-dihydroceramide or D-ribo-phytoceramide. Similar to other alkaline ceramidases, maCER1 had an alkaline pH optimum of 8.0, and it was activated by Ca2+ but inhibited by Zn2+,Cu2+, and Mn2+. maCER1 was also inhibited by sphingosine, one of its products. Metabolic labeling studies showed that overexpression of maCER1 caused a decrease in the incorporation of radiolabeled dihydrosphingosine into ceramide and complex sphingolipids but led to a concomitant increase in sphingosine-1-P (S1P) in HeLa cells. Mass...Continue Reading

References

Jan 1, 1987·Ultrastructural Pathology·P Abenoza, R K Sibley
Apr 20, 2000·The Journal of Biological Chemistry·D A Brown, E London
Apr 27, 2000·The Journal of Biological Chemistry·S El BawabY A Hannun
Sep 7, 2000·Biochemical and Biophysical Research Communications·E RomitiP Bruni
Apr 18, 2001·Molecular Pharmacology·O HwangJ D Lee
Jul 14, 2001·The Journal of Biological Chemistry·K FerlinzK Sandhoff
May 16, 2002·The Journal of Biological Chemistry·Sarah Spiegel, Sheldon Milstien
May 16, 2002·The Journal of Biological Chemistry·Yusuf A Hannun, Lina M Obeid
May 16, 2002·The Journal of Biological Chemistry·Alfred H Merrill
May 16, 2002·The Journal of Biological Chemistry·Gerrit van Meer, Quirine Lisman
Jun 1, 2002·Sub-cellular Biochemistry·Samer el BawabYasuf A Hannun
Aug 8, 2002·The Journal of Investigative Dermatology·Oliver MacheleidtKonrad Sandhoff
Oct 5, 2002·Journal of Lipid Research·Thomas DoeringKonrad Sandhoff

❮ Previous
Next ❯

Citations

Jan 14, 2005·Molecular Biology of the Cell·Wei HuCungui Mao
Dec 15, 2010·Molecular Biology of the Cell·Changqing YuanJairaj K Acharya
Mar 9, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruijuan XuCungui Mao
Mar 31, 2007·Skin Pharmacology and Physiology·E HoubenV Rogiers
Mar 2, 2012·Journal of Lipid Research·Stefka D SpassievaLina M Obeid
Jan 30, 2010·Cellular and Molecular Life Sciences : CMLS·Qiong YangCungui Mao
Sep 26, 2013·Biochimica Et Biophysica Acta·Makoto ItoMotohiro Tani
Apr 7, 2009·Biochimica Et Biophysica Acta·Bill X WuYusuf A Hannun
Jun 4, 2010·The Journal of Investigative Dermatology·Yoshikazu UchidaWalter M Holleran
Jul 30, 2015·Chemistry and Physics of Lipids·Essa M Saied, Christoph Arenz
Jun 15, 2013·Journal of Neuroendocrinology·S L PetersenJ A Lopez
May 28, 2011·British Journal of Pharmacology·Daniel CanalsYusuf A Hannun
Mar 20, 2010·Journal of Neurochemistry·Chiara MencarelliPilar Martínez-Martínez
Jun 9, 2006·Pharmacology & Therapeutics·Mazen El AlwaniYusuf A Hannun
Apr 23, 2005·Biochemical and Biophysical Research Communications·Young-ha HwangMakoto Ito
Jan 27, 2015·Journal of Bioenergetics and Biomembranes·Gauri A PatwardhanLeah J Siskind
Apr 30, 2016·The Journal of Pathology·Kifayathullah Liakath-AliLouise van der Weyden
Aug 16, 2005·Prostaglandins & Other Lipid Mediators·Michael MaceykaSarah Spiegel
Aug 20, 2015·Journal of Lipid Research·Mireia CasasampereGemma Fabrias
Aug 3, 2005·Journal of Lipid Research·Motohiro TaniYasuyuki Igarashi
Nov 17, 2017·Nutrients·Gregory H Norris, Christopher N Blesso
Aug 30, 2005·The Journal of Biological Chemistry·Motohiro TaniMakoto Ito
Oct 6, 2005·The Journal of Biological Chemistry·Susumu Mitsutake, Yasuyuki Igarashi
Dec 29, 2005·The Journal of Biological Chemistry·Mari KonoRichard L Proia
Aug 31, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruijuan XuCungui Mao
Oct 19, 2005·The Biochemical Journal·Sehamuddin GaladariYusuf A Hannun
Sep 1, 2004·The Journal of Biological Chemistry·Nathan L AldersonHiroko Hama

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.