Cloning and characterization of a new multi-stress inducible metallothionein gene in Tetrahymena pyriformis

Protist
Chengjie Fu, Wei Miao

Abstract

A new multi-stress-inducible metallothionein (MT) gene isoform has been cloned and characterized from the ciliate Tetrahymena pyriformis. Both the 5'- and 3'-UT regions of the Tp-MT2 gene are very different from the previously reported Tp-MT1 isoform in this organism and from other described MT genes in Tetrahymena pigmentosa and Tetrahymena thermophila. The putative protein sequence of Tp-MT2 contains cysteine clusters with characteristics of the typical Tetrahymena Cd-inducible MT genes. However, the sequence has a special feature of four intragenic tandem repeats within its first half, with a conserved structural pattern x(5/8)CCCx(6)CCx(6)CxCxNCxCCK. To investigate the transcriptional activities of both Tp-MT2 and Tp-MT1 genes toward heavy metals (Cd, Hg, Cu, Zn) and H(2)O(2), the mRNA levels of these two isoforms were evaluated by means of real-time quantitative PCR. Results showed that Tp-MT2 had a higher basal expression level than Tp-MT1 and both genes were induced by Cd, Hg, Cu, and Zn ions after short exposure (1h), although to different extents. Cd was the most effective metal inducer of both two isoforms, but the relative expression level of Tp-MT2 was much lower than that of Tp-MT1. Different expression patterns we...Continue Reading

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Citations

Mar 16, 2007·PloS One·Silvia DíazJuan C Gutiérrez
Jul 26, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Juan C GutiérrezA Martín-González
Jul 22, 2008·Plant Physiology and Biochemistry : PPB·Yucheng WangChuanping Yang
Dec 11, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Francesco BoldrinEster Piccinni
Jan 9, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Gianfranco SantovitoEster Piccinni
Jun 6, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Juan C GutiérrezAna Martín-González
Jan 13, 2016·Journal of Cellular Biochemistry·Muhammad Tariq ZahidAbdul Rauf Shakoori
Apr 19, 2017·International Journal of Molecular Sciences·Maria Antonietta RagusaFabrizio Gianguzza
Nov 2, 2017·Journal of Cellular Biochemistry·Huanxin ZhouWei Wang
Feb 15, 2008·Molecular Biology Reports·Rukhsana Nighat Shuja, Abdul Rauf Shakoori

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