Cloning and characterization of a novel 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Salvia miltiorrhiza involved in diterpenoid tanshinone accumulation

Journal of Plant Physiology
Zhubo DaiLuqi Huang

Abstract

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate (MVA), which is a rate-limiting step in the isoprenoid biosynthesis via the MVA pathway. In this study, the full-length cDNA encoding HMGR (designated as SmHMGR2, GenBank accession no. FJ747636) was isolated from Salvia miltiorrhiza by rapid amplification of cDNA ends (RACE). The cloned gene was then transformed into the hairy root of S. miltiorrhiza, and the enzyme activity and production of diterpenoid tanshinones and squalene were monitored. The full-length cDNA of SmHMGR2 comprises 1959bp, with a 1653-bp open reading frame encoding a 550-amino-acid protein. Molecular modeling showed that SmHMGR2 is a new HMGR with a spatial structure similar to other plant HMGRs. SmHMGR2 contains two HMG-CoA-binding motifs and two NADP(H)-binding motifs. The SmHMGR2 catalytic domain can form a homodimer. The deduced protein has an isoelectric point of 6.28 and a calculated molecular weight of approximately 58.67kDa. Sequence comparison analysis showed that SmHMGR2 had the highest homology to HMGR from Atractylodes lancea. As expected, a phylogenetic tree analysis indicates that SmHMGR2 belongs to plant HMGR group. Tissue expression pattern ...Continue Reading

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Citations

Sep 16, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Hui MaLu-Qi Huang
May 9, 2012·Biotechnology and Bioengineering·Zhubo DaiXueli Zhang
Oct 16, 2013·Metabolic Engineering·Zhubo DaiXueli Zhang
Jul 23, 2014·FEMS Yeast Research·Zhubo DaiXueli Zhang
Oct 6, 2016·Critical Reviews in Biotechnology·Ellis C O'Neill, Steven Kelly
Jan 16, 2014·Scientific Reports·Zhubo DaiXueli Zhang
Mar 30, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying YuNaixing Ye
Nov 17, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xianan ZhangLuqi Huang
Apr 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xuhong DuDongfeng Yang
Dec 13, 2017·Frontiers in Plant Science·Na YangZhezhi Wang
Nov 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huabei ZhangLuqi Huang
Oct 2, 2019·Annals of Botany·Hui WeiQiang Zhuge
Sep 13, 2011·Journal of Experimental Botany·Gabriela SotoNicolás Daniel Ayub
Apr 26, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Jin-Ping LiuLiangsheng Zhang
May 12, 2018·Planta·Swati UpadhyayRakesh Kumar Shukla
Mar 30, 2019·Frontiers in Pharmacology·Zhouqian JiangLuqi Huang
Oct 24, 2018·Plant Science : an International Journal of Experimental Plant Biology·Bingcong XingZongsuo Liang

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