Cloning and characterization of a novel binding factor (GMEB-2) of the glucocorticoid modulatory element.

The Journal of Biological Chemistry
H ZengS S Simons

Abstract

The 21-base pair glucocorticoid modulatory element (GME) of the rat tyrosine aminotransferase gene is the only cis-acting element known to modulate the transcriptional activity of receptors bound to glucocorticoid response elements. Specifically, the GME increases the activity of complexes bound both by physiological concentrations of glucocorticoids, due to a left shift in the dose-response curve, and by saturating concentrations of anti-glucocorticoids. For this reason, the nuclear protein(s) that has been demonstrated to bind to the GME is of major interest as a possible transcription factor with hitherto undescribed properties. Subsequent studies indicated that not one but two proteins of 88 and 67 kDa (= GMEB-1 and -2, respectively) formed a heteromeric complex with double-stranded GME oligonucleotides in gel shift assays and participated in the expression of GME activity (Oshima, H., Szapary, D., and Simons, S. S., Jr. (1995) J. Biol. Chem. 270, 21893-21910). Here, we report the use of polymerase chain reaction of degenerate oligonucleotides and 5'- and 3'-rapid amplification of cDNA ends to clone two cDNAs of 2. 0 and 1.9 kilobase pairs that probably result from alternative splicing. Both cDNAs encoded open reading frame...Continue Reading

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Citations

Jul 12, 2002·Genesis : the Journal of Genetics and Development·Alexey VeraksaWilliam McGinnis
May 28, 2003·Trends in Pharmacological Sciences·S Stoney Simons
May 29, 2003·The Journal of Nutritional Biochemistry·Huawei ZengJohn W Finley
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kathleen A ClarkCarol C Gregorio
May 10, 2012·PLoS Genetics·Madhura KulkarniHarold E Smith
Jan 29, 2002·The Journal of Biological Chemistry·Sunil KaulS Stoney Simons
Jun 1, 2002·Molecular Endocrinology·G Alexander AbelTheo Rein
Apr 2, 2002·The Journal of Nutrition·Huawei Zeng

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