PMID: 11690647Nov 3, 2001Paper

Cloning and characterization of a novel gene encoding L-ribose isomerase from Acinetobacter sp. strain DL-28 in Escherichia coli

Biochimica Et Biophysica Acta
R M MizanurKen Izumori

Abstract

The gene encoding a novel L-ribose isomerase (L-RI) from Acinetobacter sp. was cloned into Escherichia coli and nucleotide sequence was determined. The gene corresponded to an open reading frame of 747 bp that codes for a deduced protein of 249 amino acids, which showed no amino acid sequence similarity with any other sugar isomerases. After expression of the gene in E. coli using pUC118 the recombinant L-RI was purified to homogeneity using different chromatographic methods. The overall enzymatic properties of the purified recombinant L-RI were the same as those of the authentic L-RI. To our knowledge, this is the first time report concerning the L-RI gene.

References

Aug 4, 2005·Journal of Bacteriology·Jesse S WrightRichard P Novick
Oct 20, 2005·Journal of Bioscience and Bioengineering·Z AhmedKen Izumori

Citations

Jan 20, 2009·Applied Microbiology and Biotechnology·M HelantoA Nyyssölä
Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Koen BeerensWim Soetaert
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Xingding Zhou, Jin Chuan Wu
Dec 26, 2006·Journal of Bacteriology·Eun-Ah ChoYu-Ryang Pyun
Sep 18, 2007·Applied and Environmental Microbiology·M HelantoA Nyyssölä
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hiromi YoshidaShigehiro Kamitori
Dec 5, 2012·Journal of Bioscience and Bioengineering·Kenji MorimotoKen Izumori
Jun 15, 2010·Journal of Bioscience and Bioengineering·Hyun-Jung KwonDeok-Kun Oh
Jun 3, 2015·Journal of Bioscience and Bioengineering·Sirinan ShompoosangKenji Morimoto
May 29, 2015·Bioscience, Biotechnology, and Biochemistry·Yuji TeramiGoro Takata
Jul 10, 2010·Journal of Molecular Biology·Laura M van StaalduinenZongchao Jia
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Sirinan ShompoosangKenji Morimoto
Jul 4, 2018·Applied Microbiology and Biotechnology·Zheng FangWanmeng Mu
Jun 26, 2020·The Journal of Microbiology·Soo-Jung KimSoo-Jin Yeom
Jun 4, 2020·Journal of Agricultural and Food Chemistry·Zijie LiXiao-Dong Gao
Jun 12, 2021·Food Research International·Shahid MahmoodWanmeng Mu

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