Cloning and characterization of a novel LpWRKY1 transcription factor in tomato

Plant Physiology and Biochemistry : PPB
Markus Georg HofmannThomas Roitsch

Abstract

The initiation of defence responses in plants is accompanied by fundamental changes in gene expression: the expression of pathogenesis-related genes is co-ordinately regulated with metabolic changes such as down regulation of photosynthesis and induction of sink metabolism. To identify candidate regulators of this co-ordinated regulatory mechanism, the role of WRKY transcription factors in the initiation of defence response was analysed in tomato. A WRKY-type transcription factor (LpWRKY1) from tomato was cloned by a reverse Northern approach. The corresponding mRNA is rapidly and transiently induced after challenging the cells with an elicitor-preparation derived from the wilt inducing fungus Fusarium oxysporum lycopersici (E-FOL) and the fungal elicitor chitosan, whereas the endogenous signals systemin and salicylic acid are inactive. Inhibition of protein biosynthesis by cycloheximide results in sustained induction of mRNA for LpWRKY1. In contrast, the transient induction of the gene encoding LpWRKY1 in response to elicitation by E-FOL is inhibited by the protein-kinase inhibitor staurosporine and may be mimicked by the phosphatase inhibitors endothall and cantharidine indicating the involvement of protein phosphorylation in...Continue Reading

References

Aug 7, 1992·Cell·T Hunter, M Karin
May 1, 1997·The Plant Cell·S Zhang, D F Klessig
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·I MeskieneH Hirt
Mar 6, 1999·Current Opinion in Plant Biology·P J Rushton, I E Somssich
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Laurent DeslandesYves Marco
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
May 10, 2002·Genes & Development·Silke Robatzek, Imre E Somssich
Sep 1, 2004·Current Opinion in Plant Biology·Bekir Ulker, Imre E Somssich
Nov 1, 2005·Molecular Plant-microbe Interactions : MPMI·Frank L H MenkeDaniel F Klessig
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I E SomssichK Hahlbrock
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Jing LiE Tapio Palva
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Zuyu ZhengTesfaye Mengiste
Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Colleen KnothThomas Eulgem
Jul 24, 2007·Current Opinion in Plant Biology·Thomas Eulgem, Imre E Somssich

❮ Previous
Next ❯

Citations

Sep 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Bo LiuFeng-Ming Song
Oct 15, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S WelterP Franken
May 18, 2016·PloS One·Alessandra GhianiMaria Manuela Rigano

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.