Cloning and characterization of a novel upstream untranslated exon of the mouse Fgf-1 gene

Gene
K V HackshawI M Chiu

Abstract

Fibroblast growth factor 1 (FGF-1 or aFGF), is the prototype member of the heparin-binding growth factors which are capable of angiogenesis in vivo. FGF-1 has been implicated in atherosclerosis, cancer, wound repair and inflammatory autoimmune diseases. As part of an effort to understand the role of FGF-1 in the etiopathogenesis of inflammation and cancer, we have undertaken steps to isolate and characterize the mouse Fgf-1 gene. Southern blotting and sequence analysis displayed considerable conservation within the coding and upstream untranslated regions of Fgf-1 in human, mouse, hamster, rat and bovine. By using primers derived from the 5'-untranslated exon of a rat prostate-specific Fgf-1 cDNA, a 220-bp product was amplified from mouse genomic DNA via PCR. Sequence analysis of this amplicon showed that there was 80% similarity with the corresponding region of the rat FGF-cDNA sequence. Primers designed from this amplicon and the Fgf-1 coding region were used to isolate multiple overlapping genomic clones spanning the entire mouse Fgf-1 gene. Sequencing analysis of the genomic sequence upstream from this novel 5'-untranslated exon did not reveal typical TATA, CCAAT sequences. It appears that the occurrence of multiple untrans...Continue Reading

References

Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
Apr 11, 1989·Nucleic Acids Research·S P GoodrichP E Mansson
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Feb 11, 1993·Nucleic Acids Research·R A PaysonI M Chiu
Apr 7, 1995·The Journal of Biological Chemistry·R L MyersI M Chiu

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