PMID: 11322885Apr 27, 2001Paper

Cloning and characterization of a novel serine/threonine protein kinase gene expressed predominantly in developing brain

European Journal of Biochemistry
K NaraY Nagai

Abstract

We have isolated a rat gene, sbk, that encodes a novel serine/threonine protein kinase possessing a consensus sequence for an SH3-binding domain from developing rat brain. Rat SBK comprises 417 amino-acid residues consisting of a serine/threonine protein kinase consensus sequence followed by a C-terminal proline-rich region. Sequence comparison with other known kinases revealed that sbk belongs to a novel family of serine/threonine protein kinases structurally related to a Xenopus gastrula-specific protein kinase, Pk9.7. An in vitro kinase assay demonstrated that the SBK protein autophosphorylates at serine/threonine residues. Transcripts of sbk were strongly detected in brain, and the distribution shows an association with neurons but not glial cells. A marked increase in sbk transcripts was observed in developing brain in the late embryonic stage when dramatic neuronal proliferation, migration, and maturation occur. Fluorescence in situ hybridization analysis was used to map sbk to mouse chromosome 7F1-F3 and rat chromosome 1q21. These data suggest a role for SBK in signal-transduction pathways related to the control of brain development.

References

Jan 1, 1992·Cytogenetics and Cell Genetics·Y MatsudaV M Chapman
Mar 1, 1992·Molecular and Cellular Biology·R H ChenJ Blenis
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jan 1, 1989·Cytogenetics and Cell Genetics·H SatohM Sasaki
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
May 1, 1995·Trends in Biochemical Sciences·C J Sherr
Feb 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J YamadaT Serikawa
Feb 1, 1994·Scandinavian Journal of Immunology·T A NiewoldE Gruys
Nov 21, 1997·Nature·B InbalA Kimchi
Aug 12, 1998·Journal of Molecular Neuroscience : MN·D HevroniY Citri
Sep 3, 1998·The Journal of Biological Chemistry·A KatoK Inokuchi
Jan 23, 1999·The Journal of Biological Chemistry·M A SilvermanR Seger
Mar 27, 1999·The Journal of Biological Chemistry·A HausserF J Johannes
Nov 24, 1999·FEBS Letters·F J JohannesK Pfizenmaier
May 10, 2000·Current Biology : CB·R Homayouni, T Curran

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.