Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (OsAPT2) and its association with TGMS

Plant Molecular Biology
Chun-Jiang ZhouBin Wang

Abstract

A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-PCR analysis indicated that the OsAPT2 transcript in the young panicles of 'Annong S-1' is down-regulated at 29 degrees C, the critical temperature for induction of 'Annong S-1' fertility conversion. Since the panicle is likely the thermo-sensitive organ at the early stages of pollen fertility alternation, the observed heat-induced change in the OsAPT2 expression pattern in young panicles may mediate, at least in part, thermo-sensitive genic male sterility (TGMS) in 'Annong S-1'. An antisense strategy was used to suppress the expression of the OsAPT2 homolog in Arabidopsis, and the obtained homozygous transgenic plants contained lower AMP content, displayed lower pollen germination rates and exhibited some abnormalities in leaf phenotypes and flowering timing. These data suggest that OsAPT2 is likely to be involved in TGMS in the rice line 'Annong S-1'.

References

Jan 15, 1980·The Biochemical Journal·J Barankiewicz, J Paszkowski
Jun 27, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y G WangB Wang
Dec 9, 2003·Journal of Plant Physiology·Claudio StasollaHiroshi Ashihara
Dec 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S R McCouchS D Tanksley

❮ Previous
Next ❯

Citations

Jan 20, 2012·Molecular Genetics and Genomics : MGG·Iris FechterReinhard Töpfer
May 30, 2008·Plant Biotechnology Journal·Sangam DwivediRodomiro Ortiz
Jun 3, 2011·Journal of Zhejiang University. Science. B·Yuan-fei ZhouQing-zhong Xue
May 9, 2008·DNA Sequence : the Journal of DNA Sequencing and Mapping·Suowei WuBin Wang
Mar 15, 2020·International Journal of Molecular Sciences·Limiao JiangPrakash P Kumar
Jan 8, 2018·Phytochemistry·Hiroshi AshiharaAlan Crozier
Nov 21, 2013·Journal of Proteome Research·Cinthya Mirella PachecoTercilio Calsa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.