Cloning and characterization of a senescence inducing and class II tumor suppressor gene in ovarian carcinoma at chromosome region 6q27

Oncogene
F AcquatiR Taramelli

Abstract

Cytogenetic, molecular and functional analysis has shown that chromosome region 6q27 harbors a senescence inducing gene and a tumor suppressor gene involved in several solid and hematologic malignancies. We have cloned at 6q27 and characterized the RNASE6PL gene which belongs to a family of cytoplasmic RNases highly conserved from plants, to man. Analysis of 55 primary ovarian tumors and several ovarian tumor cell lines indicated that the RNASE6PL gene is not mutated in tumor tissues, but its expression is significantly reduced in 30% of primary ovarian tumors and in 75% of ovarian tumor cell lines. The promoter region of the gene was unaffected in tumors cell lines. Transfection of RNASE6PL cDNA into HEY4 and SG10G ovarian tumor cell lines suppressed tumorigenicity in nude mice. When tumors were induced by RNASE6PL-transfected cells, they completely lacked expression of RNASE6PL cDNA. Tumorigenicity was suppressed also in RNASE6PL-transfected pRPcT1/H6cl2T cells, derived from a human/mouse monochromosomic hybrid carrying a human chromosome 6 deleted at 6q27. Moreover, 63.6% of HEY4 clones and 42.8% of the clones of XP12ROSV, a Xeroderma pigmentosum SV40-immortalized cell line, transfected with RNASE6PL cDNA, developed a marked...Continue Reading

References

Oct 1, 1990·Biochemical Society Transactions·G R WallaceJ M Kelly
May 1, 1988·Cell and Tissue Kinetics·Z DarzynkiewiczK Shogen
Aug 1, 1985·Applied Biochemistry and Biotechnology·R J Linhardt
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Apr 1, 1995·British Journal of Cancer·V OrphanosJ M Boyle
Jan 1, 1995·Pediatric Radiology·L GuibaudV A Tran-Minh
Jan 1, 1995·The Journal of Clinical Endocrinology and Metabolism·L A KaneS N Thibodeau
Mar 1, 1993·British Journal of Cancer·W D FoulkesJ Trowsdale
Sep 1, 1995·Genes, Chromosomes & Cancer·L QueimadoS Castedo
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Jan 1, 1997·British Journal of Cancer·M G TibilettiC Capella
Aug 1, 1997·Nature Genetics·D Haber, E Harlow
Oct 8, 1998·The Biochemical Journal·J H EllisS Cooksley
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y YuR C Bast
Feb 13, 1999·Nature Genetics·P A Jones, P W Laird
Feb 27, 1999·Experimental Cell Research·M R SmithS M Rybak
May 20, 1999·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K MathiakW Köpp
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Oct 5, 2001·Journal of the National Cancer Institute·S C MokD W Cramer

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Citations

Jun 5, 2002·Mechanisms of Ageing and Development·Kaoru TominagaOlivia M Pereira-Smith
Sep 10, 2003·Seminars in Cancer Biology·Andreas a ViardotMartin Bentz
Dec 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Francesco AcquatiRoberto Taramelli
May 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Francesco AcquatiRoberto Taramelli
Jul 22, 2009·BMC Evolutionary Biology·Melissa S HillwigGustavo C MacIntosh
Oct 14, 2011·Viruses·Nicholas PolakowskiIsabelle Lemasson
Nov 26, 2003·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Brenda C Y LiS C Sydney Chung
Oct 31, 2002·American Journal of Medical Genetics·Nancy Wang
Jun 13, 2015·The Journal of Investigative Dermatology. Symposium Proceedings·Qianqian WangLeihong Xiang
Mar 2, 2010·Trends in Biochemical Sciences·Natalie Luhtala, Roy Parker
May 13, 2003·International Journal of Cancer. Journal International Du Cancer·Mark van DuinPeter J F Snijders
Dec 23, 2011·Biology of the Cell·Laura VidalinoPaolo Macchi
Mar 11, 2006·Cancer Genetics and Cytogenetics·M TaborelliCarlo Capella
Apr 20, 2006·Archives of Biochemistry and Biophysics·Paola CampomenosiRoberto Taramelli
Apr 27, 2005·Biochemical and Biophysical Research Communications·Shun-Yuan JiangTsu-Chung Chang
Aug 27, 2016·International Journal of Molecular Sciences·Francesca RoggianiAntonella Tomassetti
Nov 28, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andreas ViardotMartin Bentz
Oct 24, 2003·Nature·A J MungallS Beck
Feb 22, 2002·Oncogene·Alexandra SullivanTim Crook
Mar 27, 2020·Frontiers in Immunology·Nicolò BaranziniAnnalisa Grimaldi
Mar 26, 2016·Oncoscience·Levava RoizBetty Schwartz
Sep 10, 2020·Frontiers in Immunology·Lei WuYongsheng Li
Nov 22, 2019·Frontiers in Immunology·Francesco AcquatiDouglas M Noonan
Nov 15, 2016·International Journal of Oncology·Giovanna TurconiFrancesco Acquati
Oct 19, 2010·European Journal of Ophthalmology·Edoardo TartagliaCiro Costagliola
Jul 25, 2021·International Journal of Molecular Sciences·Sara MonaciAntonella Naldini

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