Cloning and characterization of a sialidase from the filamentous fungus, Aspergillus fumigatus.

Glycoconjugate Journal
Mark L WarwasMargo M Moore

Abstract

A gene encoding a putative sialidase was identified in the genome of the opportunistic fungal pathogen, Aspergillus fumigatus. Computational analysis showed that this protein has Asp box and FRIP domains, it was predicted to have an extracellular localization, and a mass of 42 kDa, all of which are characteristics of sialidases. Structural modeling predicted a canonical 6-bladed beta-propeller structure with the model's highly conserved catalytic residues aligning well with those of an experimentally determined sialidase structure. The gene encoding the putative Af sialidase was cloned and expressed in Escherichia coli. Enzymatic characterization found that the enzyme was able to cleave the synthetic sialic acid substrate, 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid (MUN), and had a pH optimum of 3.5. Further kinetic characterization using 4-methylumbelliferyl alpha-D-N-acetylneuraminylgalactopyranoside revealed that Af sialidase preferred alpha2-3-linked sialic acids over the alpha2-6 isomers. No trans-sialidase activity was detected. qPCR studies showed that exposure to MEM plus human serum induced expression. Purified Af sialidase released sialic acid from diverse substrates such as mucin, fetuin, epithelial cell gl...Continue Reading

References

Sep 1, 1976·Analytica Chimica Acta·G G Guilbault, A Iwase
Feb 1, 1992·Infection and Immunity·J E GalenJ B Kaper
Jan 1, 1989·Glycoconjugate Journal·P RoggentinR Schauer
Jun 18, 1974·Biochimica Et Biophysica Acta·Y UchidaT Sugimori
Aug 1, 1995·Diabetes Care·J C PickupA P Fitzgerald
Apr 16, 1993·Science·P O WainrightS K Stickel
Apr 25, 1996·Biochemical and Biophysical Research Communications·K Sato, T Miyagi
Jun 1, 1996·Protein Expression and Purification·S KruseR Schauer
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 1, 1997·Journal of Clinical Pathology·M CrookJ Rymer
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Nov 24, 1999·Glycobiology·E MontiG Borsani
Feb 24, 2001·Ryōikibetsu shōkōgun shirīzu·M Iwata, H Nunoi
Mar 27, 2001·Protein Science : a Publication of the Protein Society·R R CopleyC P Ponting
May 5, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K E Achyuthan, A M Achyuthan
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 16, 2002·Bioinformatics·Hideo BannaiSatoru Miyano
Mar 28, 2002·Applied and Environmental Microbiology·Brian G SheltonGeorge K Morris
Aug 9, 2002·The New England Journal of Medicine·Raoul HerbrechtUNKNOWN Invasive Fungal Infections Group of the European Organisation for Research and Treatment of Cancer and the Global Aspergillu
Oct 29, 2002·Current Biology : CB·B F LangG Burger
Jan 10, 2003·Nucleic Acids Research·V MatysE Wingender
Jan 22, 2003·Transplant Infectious Disease : an Official Journal of the Transplantation Society·A MinariS M Gordon
Jun 20, 2003·Glycoconjugate Journal·Marcio L RodriguesLuiz R Travassos
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eric R VimrSusan M Steenbergen
Apr 20, 2004·Glycoconjugate Journal·Taeko MiyagiKeiko Hata
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Oct 9, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kieren A MarrLawrence Corey
Mar 4, 2005·Biological & Pharmaceutical Bulletin·Yasuo Suzuki
Jul 25, 2006·Nature Methods·Amir AharoniStephen G Withers

❮ Previous
Next ❯

Citations

Jun 19, 2012·Fish & Shellfish Immunology·Ren-ping JinLi Sun
Feb 2, 2012·International Journal of Medical Microbiology : IJMM·Takahito ToyotomeKatsuhiko Kamei
Jul 13, 2016·Proteomics·Ismail A Ismail, Amanda J Able
Jan 11, 2016·Biotechnology and Applied Biochemistry·Xuedong WangLong Liu
Aug 20, 2020·Applied Microbiology and Biotechnology·Josman VelascoFernando Segato
Sep 28, 2020·Glycoconjugate Journal·Kazuhiro ShiozakiRyuta Wakata
Apr 30, 2021·Fungal Biology·Radoslav AbrashevMaria Angelova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.