Cloning and characterization of CAST transcript 2 and 4 in sheep

Yi chuan = Hereditas
Ju ZHANGHong-Bin LI

Abstract

As an endogenous inhibitor of the calpain system activated by Ca2+, calpastatin (CAST) plays a regulatory role in muscle proteolysis. Based on the bovine mRNA sequences, part of cDNA fragments of sheep CAST transcript 2 and 4 were obtained by RT-PCR. Bioinformatic analysis showed that sheep CAST transcript 2 was 4 358 bp in length with an open reading frame (ORF) 2 361 bp long and encoded 786 amino acids, while sheep CAST transcript 4 was 1 467 bp in length with 1 317 bp ORF encoding 438 amino acids. It was predicted that CAST type II contained four conserved domains and CAST type IV contained three conserved domains, and their secondary structures were rich in both hydrophobic regions and helical regions, with certain conserved phosphorylation sites and phosphorylation sites of protein kinase C (PKC). RT-PCR was conducted to analyze the expression patterns of CAST transcript 2 and transcript 4. CAST transcript 2 was ex-pressed in ten tissues detected while CAST transcript 4 only in testis.

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K S LeeG Lynch
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y EmoriK Suzuki
Jun 15, 1997·Archives of Biochemistry and Biophysics·F ChenL M Demers
Jun 13, 1998·The Journal of Cell Biology·D A PotterI M Herman
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y M Patel, M D Lane
Jun 1, 1999·FEBS Letters·M AvernaS Pontremoli
Jul 15, 1999·Biochemical and Biophysical Research Communications·J TakanoM Maki
Apr 12, 2005·Archives of Biochemistry and Biophysics·Peggy RaynaudValérie Amarger

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Citations

Nov 26, 2010·Molecular Biology Reports·Dai Hua WangCheng Li Zheng

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