Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I

Gene
J W KwakM H Han

Abstract

We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (gamma1, kappa), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse H-chain subgroup II(A) and kappa L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·P S Bachorik, P O Kwiterovich
Feb 1, 1995·Protein Science : a Publication of the Protein Society·L Harris, J Bajorath

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Citations

May 8, 2014·PloS One·Ju-Won KwakJoon Won Park

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