Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex

The Journal of Biological Chemistry
J A UrsittiR J Bloch

Abstract

We used degenerate primers for the amino- and carboxyl-terminal ends of the rod domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and clone cytokeratins 8 and 19 (K8 and K19) from cardiac muscle of the adult rat. Northern blots showed that K8 has a 2.2-kb transcript and K19 has a 1.9-kb transcript in both adult cardiac and skeletal muscles. Immunolocalization of the cytokeratins in adult cardiac muscle with isoform-specific antibodies for K8 and K19 showed labeling at Z-lines within the muscle fibers and at Z-line and M-line domains at costameres at the sarcolemmal membrane. Dystrophin and K19 could be co-immunoprecipitated and co-purified from extracts of cardiac muscle, suggesting a link between the cytokeratins and the dystrophin-based cytoskeleton at the sarcolemma. Furthermore, transfection experiments indicate that K8 and K19 may associate with dystrophin through a specific interaction with its actin-binding domain. Consistent with this observation, the cytokeratins are disrupted at the sarcolemmal membrane of skeletal muscle of the mdx mouse that lacks dystrophin. Together these results indicate that at least two cytokeratins are expressed in adult striated muscle, where they ...Continue Reading

References

Jun 1, 1991·The Journal of Cell Biology·M B McCormickE Fuchs
Sep 1, 1968·The Journal of Cell Biology·H IshikawaH Holtzer
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W J Nelson, E Lazarides
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W J Nelson, E Lazarides
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J V PardoS W Craig
Jan 5, 1995·Biochemical and Biophysical Research Communications·L A LowthertM B Omary
Jan 1, 1994·Annual Review of Biochemistry·E Fuchs, K Weber
Sep 1, 1994·Developmental Biology·A M KachinskyJ B Miller
Sep 1, 1996·The Journal of Cell Biology·D J MilnerY Capetanaki
Mar 14, 1998·Molecular Biology of the Cell·D ZhouR J Bloch
Jul 2, 1999·Experimental Cell Research·P MacioceR K Liem
Mar 15, 2000·Muscle & Nerve·M W WilliamsR J Bloch
Sep 26, 2000·American Journal of Physiology. Cell Physiology·M SamR L Lieber
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y MizunoL M Kunkel
Oct 24, 2002·Clinical Orthopaedics and Related Research·Robert J BlochJeanine A Ursitti
Mar 1, 2003·Histochemistry and Cell Biology·Takao HijikataHiroshi Yorifuji
Mar 5, 2003·Cellular and Molecular Life Sciences : CMLS·J KirfelJ Reichelt
May 14, 2003·Journal of Molecular Biology·Andrew J Sutherland-SmithWilliam Lehman

❮ Previous
Next ❯

Citations

Oct 11, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Linda M LundMeredith Bond
Jul 19, 2005·Genes & Development·Xiaoxia WangSteven J Burden
May 18, 2012·American Journal of Physiology. Cell Physiology·Mariah H GoodallRichard M Lovering
Jan 31, 2012·Journal of Biomedicine & Biotechnology·Sameer B ShahRobert J Bloch
Jul 10, 2009·The Journal of Clinical Investigation·M Bishr OmaryShinichiro Hanada
May 19, 2007·Experimental Cell Research·Yassemi CapetanakiStelios Psarras
Jul 30, 2005·Neuromuscular Disorders : NMD·Patrick Reed, Robert J Bloch
Aug 19, 2015·Nature Medicine·Stamatis PapathanasiouYassemi Capetanaki
Jan 10, 2012·Journal of Molecular Biology·Davin M HendersonJames M Ervasti
Nov 2, 2014·Cell and Tissue Research·Karim HniaJocelyn Laporte
Jul 11, 2006·Biochimica Et Biophysica Acta·James M Ervasti
Feb 13, 2014·Medical Molecular Morphology·Astrid Feinisa KhairaniHiroshi Yorifuji
Feb 19, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O A BuneevaA E Medvedev
Nov 14, 2019·American Journal of Physiology. Cell Physiology·Joaquin M MurielRobert J Bloch
Jan 9, 2015·American Journal of Physiology. Cell Physiology·Karla P García-PelagioRobert J Bloch
Feb 12, 2011·Journal of Muscle Research and Cell Motility·K P García-PelagioH González-Serratos
Feb 14, 2021·European Heart Journal·Rosa B ThorolfsdottirUNKNOWN DBDS Genomic Consortium
Nov 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marika SjöqvistCecilia Sahlgren
Feb 18, 2021·Investigative Ophthalmology & Visual Science·Jing-Xia Liu, Fatima Pedrosa Domellöf
Sep 24, 2021·Pflügers Archiv : European journal of physiology·Kay Ohlendieck, Dieter Swandulla

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.