Cloning and characterization of femA and femB from Staphylococcus epidermidis

Gene
W E AlbornP L Skatrud

Abstract

A DNA fragment was identified and cloned from Staphylococcus epidermidis (Se) using femA from S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to femA and femB of Sa. The genomic arrangement of the Se femA/B complex was nearly identical to that observed in Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis.

References

Apr 1, 1994·Antimicrobial Agents and Chemotherapy·C RyffelB Berger-Bächi

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Citations

Apr 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·O G Brakstad, J A Maeland
Nov 19, 2008·Biochimie·Stamatina GiannouliConstantinos Stathopoulos
Jan 21, 2012·Wiley Interdisciplinary Reviews. RNA·Kiley Dare, Michael Ibba
Jul 5, 2005·Expert Opinion on Investigational Drugs·H LabischinskiB Wieland
Aug 29, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Carbon
Jun 7, 2008·The Journal of International Medical Research·X LiT Zhou
Apr 29, 1998·Antimicrobial Agents and Chemotherapy·B Ling, B Berger-Bächi

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