PMID: 12770711May 29, 2003Paper

Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus

FEMS Microbiology Letters
Shinpei BannoIsamu Yamaguchi

Abstract

We isolated promoters of 12 genes from the rice blast fungus based on the sequences of randomly selected expressed sequence tags (ESTs) (appressorium formation stage cDNA library of Magnaporthe available from GenBank). These promoters (and the 5' coding regions if any) were fused in frame with egfp, and their expression patterns were examined under the epifluorescence microscope. Among them, two turned out to be specifically active in structures necessary for infection, viz. a promoter of adenylate cyclase interacting protein 1-like gene expressed in conidia, germ tubes, and appressoria, and a promoter of putative membrane-associated or secreted protein gene specifically expressed in appressoria. Although targeted knockout mutants of either gene failed to show detectable phenotypic alterations under laboratory conditions, these ESTs should be useful for identification of genes expressed during infection stages.

References

Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W ThatcherW J Dickinson
Aug 10, 2000·Nucleic Acids Research·K AkiyamaH Sasai

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Citations

Jan 6, 2005·Journal of Zhejiang University. Science. B·Jian-ping LuFu-cheng Lin
Feb 3, 2007·Journal of Zhejiang University. Science. B·Qing-chao JinDe-bao Li
Feb 2, 2010·The Journal of General and Applied Microbiology·Masumi IzawaTakashi Kamakura
Oct 25, 2016·The Journal of General and Applied Microbiology·Akira TanakaNaoko Takahashi-Ando
May 8, 2008·Bioscience, Biotechnology, and Biochemistry·Ken-ichiro SaitohYoko Nishizawa

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