Cloning and characterization of Gu/RH-II binding protein

Biochemical and Biophysical Research Communications
B C ValdezH Busch

Abstract

Gu/RNA helicase II (Gu/RH-II) is the first reported mammalian nucleolar RNA helicase that is a member of the D-E-A-D (Asp-Glu-Ala-Asp) box family of proteins. It has an ATP-dependent RNA unwinding (helicase) activity and a separate RNA folding activity (introduction of intramolecular secondary structure into single-stranded RNA). To determine which proteins may bind to Gu/RH-II, a yeast two-hybrid system was used. A cDNA which encoded a protein, called Gu/RH-II binding protein or GBP, was isolated and sequenced. The GBP protein is localized to the nucleus in speckled or diffuse nucleoplasmic patterns. The GBP mRNA level is highest in testis, 9- to 49-fold greater than other tissues. When GBP interacts with Gu/RH-II, proteolytic cleavage of Gu/RH-II occurs; the amino-terminal portion of Gu/RH-II is critical for this proteolysis.

Citations

Apr 6, 2000·Molecular Biology of the Cell·R F ZirwesM S Schmidt-Zachmann
Jun 1, 2004·Molecular Biology of the Cell·Noriko SaitohDavid L Spector
Aug 27, 2005·Molecular Biology of the Cell·Joana M P DesterroMaria Carmo-Fonseca
Jun 22, 2002·Molecular and Cellular Biology·Noora KotajaJorma J Palvimo
Aug 10, 2000·The Journal of Clinical Investigation·T J Kelley, H L Elmer
Nov 10, 2000·Biochemical and Biophysical Research Communications·A JunichoA Muraguchi
Sep 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·María Isabel Tussié-LunaAnanda L Roy
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·B LiuK Shuai
Jun 4, 1999·Progress in Biophysics and Molecular Biology·K Shuai
Jul 28, 2005·The FEBS Journal·Hushan YangBenigno C Valdez
Feb 8, 2006·Virus Research·Shih-Tung LiuLi-Kwan Chang
Feb 24, 2006·Journal of Neuroimmunology·Eliane Corrêa-de-SantanaWilson Savino
Aug 2, 2005·Trends in Biochemical Sciences·Peter TompaLászló Buday
Dec 18, 2007·Molecular Immunology·Tanja IlmarinenIsmo Ulmanen
Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Starr, D J Hilton
Mar 1, 2000·Trends in Biochemical Sciences·L Aravind, E V Koonin
Dec 12, 2003·Nature Reviews. Immunology·Ke Shuai, Bin Liu
Jun 24, 2017·Cell Communication and Signaling : CCS·Farhad SeifMohammadali Bahar
Jun 13, 1998·The Journal of Biological Chemistry·B A WibleA M Brown
Jan 15, 2002·The Journal of Biological Chemistry·Tamar MegidishC Wilson Xu
Sep 2, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Norman E KreiselmeierThomas J Kelley
May 4, 2000·American Journal of Physiology. Cell Physiology·Y A KuryshevB A Wible
Jan 28, 1999·The Journal of Biological Chemistry·A M MoilanenJ J Palvimo

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