Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment

The Journal of Biological Chemistry
Carolin J MerkleJunjie Chen

Abstract

A growing body of evidence suggests that establishment of sister chromatid cohesion is dependent on replication fork passage over a precohesion area. In Saccharomyces cerevisiae, this process involves an alternative replication factor C (RFC) complex that contains the four small RFC subunits as well as CTF18, CTF8, and DCC1. Here, we show that an evolutionarily conserved homologous complex exists in the nucleus of human cells. We demonstrate that hCTF18, hCTF8, and hDCC1 interact with each other as well as with the p38 subunit of RFC. This alternative RFC-containing complex interacts with proliferating cell nuclear antigen but not with the Rad9/Rad1/Hus1 complex, a proliferating cell nuclear antigen-like clamp involved in the DNA damage response. hCTF18 preferentially binds chromatin during S phase, suggesting a role during replication. Our data provide evidence for the existence of an alternative RFC complex with a probable role in mammalian sister chromatid cohesion establishment.

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Citations

Jan 27, 2004·Molecular Biology of the Cell·Melanie L MayerPhilip Hieter
Jan 27, 2004·Molecular Biology of the Cell·Cheryl D WarrenForrest A Spencer
Nov 24, 2012·Nucleic Acids Research·Javier Redondo-MuñozAna C Carrera
Jun 21, 2005·Molecular and Cellular Biology·Göran O Bylund, Peter M J Burgers
Nov 8, 2012·PLoS Genetics·Karen M BerkowitzKlaus H Kaestner
Jan 2, 2013·The Journal of Cell Biology·Kyoo-young LeeKyungjae Myung
Apr 7, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasushi ShiomiToshiki Tsurimoto
Apr 18, 2018·Nature Cell Biology·Atilgan YilmazNissim Benvenisty
Mar 10, 2006·The EMBO Journal·Shin-ichiro HiragaAnne D Donaldson
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Mar 29, 2020·Biology of Reproduction·Rebecca A HoltonKaren M Berkowitz
Feb 24, 2005·DNA Repair·Shay Ben Aroya, Martin Kupiec

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