Cloning and characterization of hNAT5/hSAN: an evolutionarily conserved component of the NatA protein N-alpha-acetyltransferase complex

Gene
Thomas ArnesenJohan R Lillehaug

Abstract

The human hARD1-NATH complex, cotranslationally acetylating the alpha-amino groups of proteins, was recently described. In S. cerevisiae and D. melanogaster this NatA complex contains a third subunit, Nat5p or San, respectively. Based on phylogenetic analyses and database searches, we here describe the human homologue, hNAT5, of these proteins. RT-PCR experiments demonstrated that hNat5 mRNA was expressed in several human cell lines. The candidacy of hNAT5 as a third subunit of the hARD1-NATH complex was investigated using anti-NATH or anti-hARD1 in co-immunoprecipitation experiments followed by Mass Spectrometry analysis of tryptic peptides. Oligopeptides specific for hNAT5 were identified. This verified the expression of endogenous hNAT5 protein in human cells and also identified hNAT5 as a NATH and hARD1 interacting partner. hNAT5 localized to the cytoplasm in accordance with hNAT5-hARD1-NATH complexes playing a role in cotranslational N-alpha-acetylation. Sequence alignment revealed a high degree of similarity of the NAT5 protein between species supporting its conserved role as a part of the complex. The predicted acetyltransferase domain within hNAT5 suggested that this protein, like hARD1, is an enzymatically active compo...Continue Reading

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Citations

Mar 7, 2012·Oncogene·T V Kalvik, T Arnesen
Sep 12, 2009·The Journal of Biological Chemistry·Rune EvjenthJohan R Lillehaug
Oct 9, 2012·Molecular & Cellular Proteomics : MCP·Håvard FoynThomas Arnesen
Jun 30, 2009·Anti-cancer Drugs·Kazuko TakuboGoshi Shiota
Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sang Hee HanSe Won Suh
Aug 8, 2009·BMC Proceedings·Kristian K StarheimThomas Arnesen
Feb 11, 2016·Scientific Reports·Om Singh RathoreRui Gonçalo Martinho
Mar 13, 2012·Trends in Biochemical Sciences·Kristian K StarheimThomas Arnesen
May 13, 2008·Trends in Genetics : TIG·Paul P Gardner, Jeppe Vinther
Jul 9, 2011·The FEBS Journal·Petra Van DammeKris Gevaert
Sep 20, 2008·Developmental Biology·Ana Pimenta-MarquesRui Gonçalo Martinho
Dec 10, 2014·Human Molecular Genetics·Line M MyklebustThomas Arnesen
Jun 15, 2016·Biochimica Et Biophysica Acta·Adrian DrazicThomas Arnesen
Dec 21, 2016·Scientific Reports·Ana Luisa RibeiroRui Gonçalo Martinho
Jun 7, 2017·Nature Communications·Felix Alexander WeyerIrmgard Sinning
Aug 8, 2015·Bioscience Reports·Rasmus ReeThomas Arnesen
Jul 29, 2018·Experimental & Molecular Medicine·Rasmus ReeThomas Arnesen
Feb 12, 2020·Nature Communications·Sunbin DengRonen Marmorstein
Dec 19, 2018·Nature Structural & Molecular Biology·Alexandra G KnorrRoland Beckmann
Dec 29, 2020·PLoS Computational Biology·Bojan KrtenicNathalie Reuter
Jan 6, 2021·Structure·Jonas WeidenhausenIrmgard Sinning
Dec 2, 2019·International Journal of Biological Macromolecules·Shikha SharmaYusuf Akhter
May 23, 2008·Journal of Cell Science·Hélène ParadisRobert L Gendron

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