Cloning and characterization of miRNAs from maize seedling roots under low phosphorus stress.

Molecular Biology Reports
Zhiming ZhangGuangtang Pan

Abstract

MicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. In this study, a small RNA library was constructed to identify conserved miRNAs as well as novel miRNAs in maize seedling roots under low level phosphorus stress. Twelve miRNAs were identified by high throughput sequencing of the library and subsequent analysis, two belong to conserved miRNA families (miRNA399b and miRNA156), and the remaining ten are novel and one of latter is conserved in gramineous species. Based on sequence homology, we predicted 125 potential target genes of these miRNAs and then expression patterns of 7 miRNAs were validated by semi-RT-PCR analysis. MiRNA399b, Zma-miR3, and their target genes (Zmpt1 and Zmpt2) were analyzed by real-time PCR. It is shown that both miRNA399b and Zma-miR3 are induced by low phosphorus stress and regulated by their target genes (Zmpt1 and Zmpt2). Moreover, Zma-miR3, regulated by two maize inorganic phosphate transporters as a newly identified miRNAs, would likely be directly involved in phosphate homeostasis, so was miRNA399b in Arabidopsis and rice. These results indicate that both conserved and m...Continue Reading

References

Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·U S MuchhalK G Raghothama
Jul 1, 1997·Genes & Development·Y YangD F Klessig
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 13, 2002·Plant Physiology·M Florian MetteAntonius J M Matzke
Sep 13, 2002·Plant Physiology·Athikkattuvalasu S KarthikeyanKashchandra G Raghothama
Feb 20, 2003·RNA·Victor AmbrosThomas Tuschl
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·K. G. Raghothama
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Kathleen A. Marrs
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Arnab MukhopadhyayAkhilesh K Tyagi
Jun 18, 2004·Molecular Cell·Matthew W Jones-Rhoades, David P Bartel
Jul 20, 2004·The Plant Cell·Ramanjulu Sunkar, Jian-Kang Zhu
Aug 5, 2004·Annals of Botany·John P HammondPhilip J White
Apr 5, 2005·The Plant Cell·Ramanjulu SunkarJian-Kang Zhu
Apr 6, 2005·Developmental Cell·Rebecca SchwabDetlef Weigel
Jun 10, 2005·Nature·Jun LuTodd R Golub
Jun 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nick LauterStephen P Moose
Sep 9, 2005·The Plant Journal : for Cell and Molecular Biology·Tzahi AraziDavid C Baulcombe
Jan 3, 2006·The Plant Cell·Tzyy-Jen ChiouChun-Lin Su
May 9, 2006·Plant Physiology·Rajendra BariWolf-Rüdiger Scheible
Jul 6, 2006·Journal of Experimental Botany·Erica MicaMario Enrico Pè
Mar 14, 2007·PLoS Computational Biology·Xuefeng ZhouWeixiong Zhang
Jun 9, 2007·Plant Physiology·Mesfin TesfayeCarroll P Vance
Jun 19, 2007·Trends in Plant Science·Ramanjulu SunkarJian-Kang Zhu
Mar 12, 2008·Journal of Experimental Botany·Steven NeillIan Wilson

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Citations

Jul 9, 2014·Trends in Plant Science·Tzu-Yin LiuTzyy-Jen Chiou
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Zhaoxia LiJuren Zhang
May 26, 2017·Cellular & Molecular Biology Letters·Md Mahmudul HasanXuexian Li
Oct 8, 2020·BMC Genomics·Shu-Yun ChenHsin-Mei Ku
May 7, 2021·Analytical and Bioanalytical Chemistry·Jin YangXilang Jin

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Methods Mentioned

BETA
electrophoresis
Reverse Transcription
PCR
reverse transcription PCR

Software Mentioned

Primer Express
DNAman
BLAST
Mfold
BLASTN
Primer
SOAP

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