Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein

Oncogene
K MakinoH Saya

Abstract

We have cloned a cDNA for a novel human homolog of the Drosophila discs large (dig) tumor suppressor protein, termed NE-dlg (neuronal and endocrine dig). Northern blot analysis revealed that the gene is highly expressed in neuronal and endocrine tissues. Fluorescence in situ hybridization (FISH) and radiation hybrid mapping studies localized the NE-dlg gene to chromosome Xq13. We also found that the NE-dlg gene encoded a 100 kDa protein. Immunolocalization studies using an NE-dlg antibody showed that the protein tended to be expressed in non-proliferating cells, such as neurons, cells in Langerhans islets of the pancreas, myocytes of the heart muscles, and the prickle and functional layer cells of the esophageal epithelium. Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little expression of the NE-dlg protein. In addition, yeast two-hybrid screening and in vitro binding assays revealed that the NE-dlg interacted with the carboxyl-terminal region of the APC tumor suppressor protein. These data suggest that NE-dlg negatively regulates cell proliferation through its interaction with the APC protein.

Citations

Nov 24, 1999·The Journal of Biological Chemistry·K NosakaH Ueyama
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S D DimitratosP J Bryant
May 10, 2003·Nature Cell Biology·Nathalie SansRobert J Wenthold
Dec 19, 2003·Oncogene·Lukas E DowPatrick O Humbert
Feb 3, 2005·International Journal of Cancer. Journal International Du Cancer·Harald zur Hausen, Ethel-Michele de Villiers
Mar 7, 2012·International Journal of Cancer. Journal International Du Cancer·Vanitha Krishna SubbaiahLawrence Banks
May 21, 2015·Cancer Informatics·Wan-Hsin LinPanos Z Anastasiadis
Dec 3, 1999·Journal of Neurochemistry·D G StathakisK Forsman-Semb
May 27, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Patrick HumbertHelena Richardson
Jan 2, 2014·Journal of Neuro-oncology·Zhongyu LiuChunfang Gao
Oct 10, 2003·Cancer Investigation·Dezhong Joshua LiaoFazlul H Sarkar
Oct 28, 2006·Radiation Research·Masahiro MutoKouichi Tatsumi
Oct 24, 2008·Molecular Biology of the Cell·Fabien PerugiClaudine Pique
Jan 22, 2011·Molecular Cancer Therapeutics·Eric R GamazonRong Stephanie Huang
May 16, 2007·Progress in Histochemistry and Cytochemistry·Lorenza González-MariscalErika Garay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.